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Retrovirus-mediated transduction of a cytosine deaminase gene preserves the stemness of mesenchymal stem cells.

Authors
Park, JS; Chang, DY; Kim, JH; Jung, JH; Park, J; Kim, SH; Lee, YD; Kim, SS; Suh-Kim, H
Citation
Experimental & molecular medicine, 45:e10-e10, 2013
Journal Title
Experimental & molecular medicine
ISSN
1226-36132092-6413
Abstract
Human mesenchymal stem cells (MSCs) have emerged as attractive cellular vehicles to deliver therapeutic genes for ex-vivo therapy of diverse diseases; this is, in part, because they have the capability to migrate into tumor or lesion sites. Previously, we showed that MSCs could be utilized to deliver a bacterial cytosine deaminase (CD) suicide gene to brain tumors. Here we assessed whether transduction with a retroviral vector encoding CD gene altered the stem cell property of MSCs. MSCs were transduced at passage 1 and cultivated up to passage 11. We found that proliferation and differentiation potentials, chromosomal stability and surface antigenicity of MSCs were not altered by retroviral transduction. The results indicate that retroviral vectors can be safely utilized for delivery of suicide genes to MSCs for ex-vivo therapy. We also found that a single retroviral transduction was sufficient for sustainable expression up to passage 10. The persistent expression of the transduced gene indicates that transduced MSCs provide a tractable and manageable approach for potential use in allogeneic transplantation.
MeSH terms
AdolescentAnimalsCell Death/drug effectsCell Line, TumorCell Proliferation/drug effectsCell Transformation, Neoplastic/drug effects/pathologyChildCytosine Deaminase/*genetics/therapeutic useFluorouracil/pharmacologyGenetic TherapyGenomic Instability/drug effectsHumansKaryotypeMesenchymal Stromal Cells/*cytology/drug effects/metabolismMiceMultipotent Stem Cells/cytology/drug effects/metabolismNeoplasms/therapyRetroviridae/*metabolismTime Factors*Transduction, Genetic
DOI
10.1038/emm.2013.21
PMID
23429359
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Anatomy
Journal Papers > School of Medicine / Graduate School of Medicine > Hematology-Oncology
Journal Papers > School of Medicine / Graduate School of Medicine > Neurosurgery
AJOU Authors
장, 다영박, 준성김, 세혁이, 영돈김, 성수서, 해영
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