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Wnt5a stimulates chemotactic migration and chemokine production in human neutrophils.

Authors
Jung, YS; Lee, HY; Kim, SD; Park, JS; Kim, JK; Suh, PG; Bae, YS
Citation
Experimental & molecular medicine, 45:e27-e27, 2013
Journal Title
Experimental & molecular medicine
ISSN
1226-36132092-6413
Abstract
Wnt5a is a ligand that activates the noncanonical Wnt signaling pathways (β-catenin-independent pathways). Human neutrophils expressed several Wnt5a receptors, such as Frizzled 2, 5 and 8. Stimulation of human neutrophils with Wnt5a caused chemotactic migration and the production of two important chemokines, CXCL8 and CCL2. CCL2 production by Wnt5a was mediated by a pertussis toxin-sensitive G-protein-dependent pathway. Wnt5a also stimulated the phosphorylation of three mitogen-activated protein kinases (MAPKs: ERK, p38 MAPK and JNK) and Akt. Inhibition of ERK, p38 MAPK or JNK by specific inhibitors induced a dramatic reduction in Wnt5a-induced CCL2 production. Supernatant collected from lipopolysaccharide-stimulated macrophages induced neutrophil chemotaxis, which was significantly inhibited by anti-Wnt5a antibody. Our results suggested that Wnt5a may contribute to neutrophil recruitment, mediating the inflammation response.
MeSH terms
Activating Transcription Factor 2/metabolismAnimalsCell SeparationChemokines/*biosynthesisChemotaxis/*drug effectsCulture Media, Conditioned/pharmacologyExtracellular Signal-Regulated MAP Kinases/metabolismGTP-Binding Proteins/metabolismHumansJNK Mitogen-Activated Protein Kinases/metabolismLipopolysaccharides/pharmacologyMacrophages/drug effects/metabolismMiceNF-kappa B/metabolismNeutrophils/*cytology/drug effects/enzymology/*metabolismPertussis Toxin/pharmacologyPhosphatidylinositol 3-Kinases/metabolismProto-Oncogene Proteins c-akt/metabolismReceptors, Wnt/metabolismType C Phospholipases/metabolismWnt Proteins/*pharmacologyp38 Mitogen-Activated Protein Kinases/metabolism
DOI
10.1038/emm.2013.48
PMID
23764954
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Hematology-Oncology
AJOU Authors
박, 준성
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