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A genetic effect of IL-5 receptor α polymorphism in patients with aspirin-exacerbated respiratory disease.

Authors
Losol, P; Kim, SH; Shin, YS; Ye, YM; Park, HS
Citation
Experimental & molecular medicine, 45:e14-e14, 2013
Journal Title
Experimental & molecular medicine
ISSN
1226-36132092-6413
Abstract
Persistent eosinophil activation in both the upper and lower airway mucosa is a central feature of aspirin-exacerbated respiratory disease (AERD). Eosinophil activation and survival are profoundly influenced by interleukin 5 (IL-5) and its receptor, IL-5R. In patients susceptible to allergic disorders, IL-5 receptor α (IL5RA) polymorphisms have been reported; however, an association with AERD remains unclear. We hypothesize that IL5RA polymorphisms may contribute to eosinophil activation in AERD patients. We recruited 139 AERD patients, 171 aspirin-tolerant asthma patients and 160 normal controls. IL5RA polymorphisms (-5993G>A, -5567C>G and -5091G>A) were genotyped and functional activity of polymorphism was assessed by luciferase reporter assay and electrophoretic mobility shift assay (EMSA). There was no significant difference in the genotype frequency of the three polymorphisms among the three groups. AERD patients carrying the AA genotype at -5993G>A had a significantly higher presence of serum-specific immunoglobulin E (IgE) to staphylococcal enterotoxin A (P=0.008) than those with the GG/GA genotype. In vitro, the -5993A allele had a higher promoter activity compared with the -5993G allele in human mast cell (HMC-1; P=0.030) and human promyelocytic leukemia (HL-60; P=0.013) cells. In EMSA, a -5993A probe produced a specific shifted band than the -5993G had. These findings suggest that a functional polymorphism in IL5RA may contribute to eosinophil and mast cell activation along with specific IgE responses to staphylococcal enterotoxin A in AERD patients.
MeSH terms
AdultAspirin/*adverse effectsElectrophoretic Mobility Shift AssayFemaleGene Frequency/geneticsHumansInterleukin-5 Receptor alpha Subunit/*geneticsMaleMiddle AgedPhenotypePolymorphism, Single Nucleotide/*geneticsRespiration Disorders/*chemically induced/*geneticsTranscription, Genetic
DOI
10.1038/emm.2013.24
PMID
23470716
Appears in Collections:
Journal Papers > Research Organization > Regional Clinical Trial Center
Journal Papers > School of Medicine / Graduate School of Medicine > Allergy
AJOU Authors
김, 승현신, 유섭예, 영민박, 해심
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