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Protective effect of thioredoxins 1 and 2 in retinal ganglion cells after optic nerve transection and oxidative stress.

Authors
Munemasa, Y; Kim, SH; Ahn, JH; Kwong, JM; Caprioli, J; Piri, N
Citation
Investigative ophthalmology & visual science, 49(8):3535-3543, 2008
Journal Title
Investigative ophthalmology & visual science
ISSN
0146-04041552-5783
Abstract
PURPOSE: Oxidative stress has been implicated in retinal ganglion cell (RGC) death pathways after optic nerve transection (ONT) and during glaucomatous neuropathy. The authors investigated the expression and cell-protective roles of thioredoxins (cytosolic Trx1 and mitochondrial Trx2), important regulators of the cellular redox state, on RGCs after ONT and pharmacologic oxidative stress induction.



METHODS: ONT was performed on adult Wistar rats. Trx1 and Trx2 quantitative and spatial expression were examined with Western blot and immunohistochemistry, respectively. Electroporation and calcium phosphate-mediated procedures were used to deliver Trx1 and Trx2 expression constructs to RGCs in vivo and to cultured RGC-5 cells, respectively. Cell-protective effects of Trx1 and Trx2 overexpression on RGCs after ONT and on RGC-5 cells treated with glutamate/buthionine sulfoximine (BSO) were determined by RGC density analysis and cell viability assay, respectively.



RESULTS: Upregulation of Trx1 and Trx2 was observed in RGCs at different times after ONT and in RGC-5 cells after glutamate/BSO treatment. Trx1 and Trx2 overexpression in RGC-5 cells increased their survival rate by approximately twofold and threefold 24 and 48 hours after glutamate/BSO treatment, respectively. A neuroprotective effect of Trx1 and Trx2 overexpression on RGCs was also observed in vivo; the survival rate of RGCs was increased by 35% and 135%, respectively, 1 and 2 weeks after ONT.



CONCLUSIONS: These findings provide evidence for in vitro and in vivo cell-protective effects of Trx1 and Trx2 on RGCs against oxidative stress-induced neurodegeneration.
MeSH terms
AnimalsAxotomyBlotting, WesternButhionine Sulfoximine/pharmacologyCell CountCell SurvivalElectroporationGene Expression Regulation/physiologyGlutamic Acid/pharmacologyImmunohistochemistryOptic Nerve Injuries/metabolismOptic Nerve Injuries/pathologyOptic Nerve Injuries/prevention & control*Oxidative Stress*RatsRats, WistarRetinal Ganglion Cells/drug effectsRetinal Ganglion Cells/metabolism*Thioredoxins/physiology*TransfectionUp-Regulation
DOI
10.1167/iovs.08-1716
PMID
18441302
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Ophthalmology
AJOU Authors
안, 재홍
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