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Organic Acid Profiling Analysis in Culture Media of Lactic Acid Bacteria by Gas Chromatography-Mass Spectrometry

Authors
Lee, JY | Nguyen, DT | Park, YS | Hwang KY | Cho, YS | Kang, KD | Yoon, JH | Yu, JD | Yee, ST | Ahn, YH  | Lee, G  | Seong, SI | Paik, MJ
Citation
Mass Spectrometry Letters, 3(3). : 74-77, 2012
Journal Title
Mass Spectrometry Letters
ISSN
2233-42032093-8950
Abstract
Organic acid (OA) profiling analysis was performed in culture media from Lactobacillus pentosus K34 (L. pentosus

K34) and Pediococcus lolli PL24 (P. lolli PL24) by gas chromatography-mass spectrometry (GC-MS) following methoxime/tert-butyldimethylsilyl derivatives. 12 OAs were positively identified in culture media. Most of OA levels from L. pentosus K34 of hetero lactic fermentation were found to be higher when compared with those from P. lolli PL24 of homo lactic fermentation, which may explain different OA metabolism in each strain. In addition, the distorted dodecagonal star patterns were readily distinguishable, and the characteristics of each strain were well represented. The present study demonstrates that the OA metabolic profiling method by GC-MS combined with star pattern recognition is useful for the monitoring study of characteristic OA metabolism in various microorganisms.
Keywords
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Neurosurgery
Journal Papers > School of Medicine / Graduate School of Medicine > Physiology
Ajou Authors
안, 영환  |  이, 광
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