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High-Dose 111In Induces G1 Cell Cycle Arrest and Cell Death in Rat Bone Marrow Mesenchymal Stem Cells

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dc.contributor.authorPark, BN-
dc.contributor.authorShim, W-
dc.contributor.authorAhn, YH-
dc.contributor.authorLee, JH-
dc.contributor.authorAn, YS-
dc.contributor.authorYoon, JK-
dc.date.accessioned2014-07-08-
dc.date.available2014-07-08-
dc.date.issued2012-
dc.identifier.issn1869-3474-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/10488-
dc.description.abstractPurpose: This study was performed to evaluate the effect of 111In-labeling on the cell growth, cycle and viability of bone marrow mesenchymal stem cells (BMSCs).



Methods: Rat BMSCs were labeled with various doses of 111In (0.4–11.1 Bq/cell). The growth curve of 111In-BMSCs was obtained up to 14th day of labeling. The cell cycle was evaluated by 5-bromo-2-deoxyuridine (BrdU) labeling or propidium iodide (PI) staining. Senescent cells were counted under a light microscope after staining with 5-bromo-4-chloro-3-indolyl-D-galactopyranoside. Flow cytometry was performed to measure apoptotic and necrotic fractions after staining with annexin V-FITC and PI.



Results: The growth of BMSCs labeled with higher doses of 111In (4.4 or 11.1 Bq/cell) was significantly inhibited from the 3rd day of labeling. Flow cytometry revealed less BrdU-positive BMSCs at 11.1 Bq 111In/cell during all measurement days and G1 arrest at 4.4 and 11.1 Bq 111In/cell. Significant increases in apoptosis and necrosis were also observed at 4.4 (3.04%/1.35%) and 11.1 Bq 111In/cell (9.07%/3.18%) on the 14th day (control = 1.60%/0.39%). However, no cellular senescence was visualized up to the 14th day.



Conclusion: A high dose of 111In-labeling induced cell cycle arrest and death in BMSCs; therefore, it should be used with a careful dosimetry in case of applying it to humans.
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dc.language.isoen-
dc.titleHigh-Dose 111In Induces G1 Cell Cycle Arrest and Cell Death in Rat Bone Marrow Mesenchymal Stem Cells-
dc.typeArticle-
dc.identifier.urlhttp://link.springer.com/article/10.1007/s13139-011-0124-6#-
dc.subject.keyword111In-tropolone-
dc.subject.keywordMesenchymal stem cells-
dc.subject.keywordCell cycle-
dc.subject.keywordApoptosis-
dc.subject.keywordNecrosis-
dc.contributor.affiliatedAuthor박, 복남-
dc.contributor.affiliatedAuthor안, 영환-
dc.contributor.affiliatedAuthor이, 재호-
dc.contributor.affiliatedAuthor안, 영실-
dc.contributor.affiliatedAuthor윤, 준기-
dc.type.localJournal Papers-
dc.identifier.doi10.1007%2Fs13139-011-0124-6#-
dc.citation.titleNuclear medicine and molecular imaging-
dc.citation.volume46-
dc.citation.number2-
dc.citation.date2012-
dc.citation.startPage81-
dc.citation.endPage88-
dc.identifier.bibliographicCitationNuclear medicine and molecular imaging, 46(2). : 81-88, 2012-
dc.identifier.eissn1869-3482-
dc.relation.journalidJ018693474-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Nuclear Medicine & Molecular Imaging
Journal Papers > School of Medicine / Graduate School of Medicine > Neurosurgery
Journal Papers > School of Medicine / Graduate School of Medicine > Biochemistry & Molecular Biology
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