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An improved method for a mouse focal ischemic stroke model : assessment of cerebral blood flow and functional impairment

Authors
Park, Sin Young; Marasini, Subash; kim, Geu Hee; Lee, Young-Don; Kim, Sung-Soo; Suh-Kim, Haeyoung
Department
Department of Anatomy
Abstract
Stroke is one of the commonest causes of death and disability. Despite extensive efforts, therapeutic options for improving stroke recovery remain limited due the complexity of pathophysiology, which could be unraveled with ischemic models of genetically modified mice. Here, we optimized the procedure for mouse stroke model where blood flow in the middle cerebral artery was blocked with an intraluminal suture for 60 minutes and the ischemic injury was verified by using indocyanine green (ICG). The animals exhibited the increased survivability after optimization of the occluding procedures and administration of antibiotics and consistent induction rates. On batteries of behavioral test to assess the functional impairments, animals showed the maximal severity during the first week but spontaneously recovered starting from the second week. In conclusion, we developed an improved approach which allows us to investigate the role of the cell death related molecules in the disease progression and to evaluate the modes of action of candidate drugs.
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