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Change of platelet activation markers using flow cytometry in patients with hematology/oncology disorders after transfusion.

Authors
Lim, YA; Cho, SR; Lee, WG; Park, JS; Kim, SW
Citation
Platelets, 19(5):328-334, 2008
Journal Title
Platelets
ISSN
0953-71041369-1635
Abstract
In spite of the frequent need of platelet transfusions, there is limited information on the association of platelet activation markers, in transfused patients with hematology/oncology disorders, with platelet function using flow cytometry. The goal of this study was to evaluate the changes of PAC-1 binding and CD62P expression, with or without agonists in patients after transfusions. Twenty-eight whole blood samples were obtained from 24 patients admitted to the department of Hematology & Oncology and transfused with platelets; these samples were compared to 30 healthy controls. Whole blood samples, either with or without agonists, such as 20 microM adenosine diphosphate (ADP) or 100 microM thrombin receptor activating peptide (TRAP), were stained with the fluorescein conjugated monoclonal antibodies PAC-1 or CD62P. Then, the percent expression for each marker was analysed using flow cytometry. ADP and TRAP induced an increased percentage of CD62P expression and PAC-1 binding after platelet transfusions compared to the samples studied before transfusion, and these findings were lower than those of the healthy controls. However, the expression of platelets without the agonists was not significantly changed, despite the transfusions. Therefore, agonist-induced platelet activation markers, studied by flow cytometry, appear to be more useful for the evaluation of platelet function after transfusions than platelet activation markers without agonists.
MeSH terms
Adenosine Diphosphate/pharmacologyAdolescentAdultAgedAnemia, Aplastic/blood*Anemia, Aplastic/complicationsAntineoplastic Agents/adverse effectsAntineoplastic Agents/therapeutic useBiological MarkersFemaleFlow Cytometry/methods*HumansLeukemia/blood*Leukemia/complicationsLeukemia/drug therapyMaleMiddle AgedP-Selectin/analysisPlatelet Activation*/drug effectsPlatelet Glycoprotein GPIIb-IIIa Complex/analysisPlatelet Transfusion*Receptors, Fibrinogen/analysisReceptors, ThrombinThrombocytopenia/blood*Thrombocytopenia/chemically inducedThrombocytopenia/etiologyThrombocytopenia/therapy
DOI
10.1080/09537100802129867
PMID
18791938
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Laboratory Medicine
Journal Papers > School of Medicine / Graduate School of Medicine > Hematology-Oncology
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임영애조성란이위교박준성
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