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Comparison of multiplex reverse transcription polymerase chain reaction and conventional cytogenetics as a diagnostic strategy for acute leukemia.

Authors
Park, JS; Yi, JW; Jeong, SH; Lee, HW; Kang, SY; Choi, JH; Kim, HC; Park, JE; Kim, E; Lim, YA; Kim, HJ; Cho, SR
Citation
International journal of laboratory hematology, 30(6):513-518, 2008
Journal Title
International journal of laboratory hematology
ISSN
1751-55211751-553X
Abstract
To clarify the usefulness of multiplex reverse transcription polymerase chain reaction (mRT-PCR) in diagnosing acute leukemia, mRT-PCR detecting 28 different translocations was performed on bone marrow aspirates of 156 patients with acute leukemia, and the results were compared with conventional chromosomal karyotypes. About 113 of 156 patients had acute myeloid leukemia (AML), and 36 had acute lymphoid leukemia (ALL) with patients' ages ranging from 1 to 84 (median: 34.5). Concordance rate between karyotyping and mRT-PCR was 50% (51% in AML and 44% in ALL). Karyotype revealed chromosomal abnormalities in 70 patients (45%) while mRT-PCR showed some aberrations in 59 patients (38%). mRT-PCR detected t(1;19), t(4;11), t(9;11), t(10;11), t(11;19), t(12;21), and TAL1d, which were not detected by G-banding. In addition, 10 patients with t(15;17), one patient with t(8;21), and four patients with t(9;22) detected by mRT-PCR revealed normal karyotypes. However, mRT-PCR did not detect numerical abnormalities, deletions, and translocations other than the 28 translocations included in the assay as expected. In conclusion, although it cannot be a substitute of the conventional chromosome analysis, mRT-PCR could be a complementary diagnostic strategy of acute leukemia.
MeSH terms
AdolescentAdultAgedAged, 80 and overChildChild, PreschoolHumansInfantKaryotypingLeukemia, Myeloid, Acute/diagnosis*Middle AgedPrecursor Cell Lymphoblastic Leukemia-Lymphoma/diagnosis*Reverse Transcriptase Polymerase Chain Reaction/methods*Young Adult
DOI
10.1111/j.1751-553X.2007.00992.x
PMID
18983303
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Laboratory Medicine
Journal Papers > School of Medicine / Graduate School of Medicine > Hematology-Oncology
Journal Papers > School of Medicine / Graduate School of Medicine > Pediatrics & Adolescent Medicine
Journal Papers > School of Medicine / Graduate School of Medicine > Medical Genetics
AJOU Authors
박, 준성정, 성현이, 현우강, 석윤최, 진혁김, 효철박, 준은임, 영애김, 현주조, 성란
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