Glucose biosensor in your pocket: Paper-based enzymatic colorimetry on a smartphone
Chun, Hyeong Jin; Park, Yoo Min; Han, Yong Duk; Yoon, Hyun C.
Department of Molecular Science & Technology
A paper-based glucose biosensor for the diabetes monitoring was developed. For simple quantification of analyze glucose concentration, a smartphone-based optical biosensor was introduced. In this study, a bienzymatic colorimetry using glucose oxidase (GOx), horseradish peroxidase (HRP) and chromogenic compounds was used as a biosensing principle and implanted to the paper-based analytical device (PAD) which is designed for integration with a smartphone. To make hydrophobic channel and bio-reaction chamber on the paper, the wax printing method was used. The PAD consists of top layer for loading and detection zones and bottom layer for fluidic channel. The detection zone is modified with GOx, HRP and chromogenic compound. In the presence of glucose, H2O2 was produced by GOx. Then, HRP converts the chromogenic compound into a final-product, occuring blue-color development. The developed color intensity was registered by using the digital camera of the smartphone and analyzed by the image J program. To avoid the signal interference from external light, the measurement procedure was carried out in an especially designed light-tight box which could be adapted to the smartphone. Using the developed biosensing system, various concentrations of glucose samples in human serum (5-17 mM) were quantified.
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