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Sanguinarine-induced apoptosis: generation of ROS, down-regulation of Bcl-2, c-FLIP, and synergy with TRAIL.

DC Field Value Language
dc.contributor.authorKim, S-
dc.contributor.authorLee, TJ-
dc.contributor.authorLeem, J-
dc.contributor.authorChoi, KS-
dc.contributor.authorPark, JW-
dc.contributor.authorKwon, TK-
dc.date.accessioned2011-01-14T01:49:49Z-
dc.date.available2011-01-14T01:49:49Z-
dc.date.issued2008-
dc.identifier.issn0730-2312-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/1137-
dc.description.abstractSanguinarine is a benzophenanthridine alkaloid derived from the root of Sanguinaria canadensis and other poppy-fumaria species, possessing potent antibacterial, antifungal, and anti-inflammatory activities. In this study, we investigated the underling mechanisms by which sanguinarine induce apoptosis in human breast cancer MDA-231 cells. Treatment of MDA-231 cells with sanguinarine induced remarkable apoptosis accompanying the generation of ROS. Consistently, sanguinarine-induced apoptosis was mediated by the increased reproductive cell death. Pretreatment with NAC or GSH attenuated sanguinarine-induced apoptosis, suggesting the involvement of ROS in this cell death. During sanguinarin-induced apoptosis, protein levels of pro-caspase-3, Bcl-2, cIAP2, XIAP, and c-FLIPs were reduced. Sanguinarine-mediated apoptosis was substantially blocked by ectopic expression of Bcl-2 and cFLIPs. Additionally, we found that sub-lethal doses of sanguinarine remarkably sensitized breast cancer cells to TRAIL-mediated apoptosis, but the cell death induced by sanguinarine and TRAIL in combination was not blocked by overexpression of Bcl-2 or Akt. Therefore, combinatory treatment of sanguinarine and TRAIL may overcome the resistance of breast cancer cells due to overexpression of Akt or Bcl-2.-
dc.formatapplication/pdf-
dc.language.isoen-
dc.subject.MESHAnti-Infective Agents-
dc.subject.MESHApoptosis-
dc.subject.MESHBenzophenanthridines-
dc.subject.MESHCASP8 and FADD-Like Apoptosis Regulating Protein-
dc.subject.MESHCell Line, Tumor-
dc.subject.MESHDown-Regulation-
dc.subject.MESHGene Expression Regulation, Neoplastic-
dc.subject.MESHGlutathione-
dc.subject.MESHHumans-
dc.subject.MESHIsoquinolines-
dc.subject.MESHModels, Biological-
dc.subject.MESHPlant Extracts-
dc.subject.MESHProto-Oncogene Proteins c-akt-
dc.subject.MESHProto-Oncogene Proteins c-bcl-2-
dc.subject.MESHReactive Oxygen Species-
dc.subject.MESHTNF-Related Apoptosis-Inducing Ligand-
dc.titleSanguinarine-induced apoptosis: generation of ROS, down-regulation of Bcl-2, c-FLIP, and synergy with TRAIL.-
dc.typeArticle-
dc.identifier.pmid18189268-
dc.contributor.affiliatedAuthor최, 경숙-
dc.type.localJournal Papers-
dc.identifier.doi10.1002/jcb.21672-
dc.citation.titleJournal of cellular biochemistry-
dc.citation.volume104-
dc.citation.number3-
dc.citation.date2008-
dc.citation.startPage895-
dc.citation.endPage907-
dc.identifier.bibliographicCitationJournal of cellular biochemistry, 104(3). : 895-907, 2008-
dc.identifier.eissn1097-4644-
dc.relation.journalidJ007302312-
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Journal Papers > School of Medicine / Graduate School of Medicine > Biochemistry & Molecular Biology
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