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Sanguinarine-induced apoptosis: generation of ROS, down-regulation of Bcl-2, c-FLIP, and synergy with TRAIL.
DC Field | Value | Language |
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dc.contributor.author | Kim, S | - |
dc.contributor.author | Lee, TJ | - |
dc.contributor.author | Leem, J | - |
dc.contributor.author | Choi, KS | - |
dc.contributor.author | Park, JW | - |
dc.contributor.author | Kwon, TK | - |
dc.date.accessioned | 2011-01-14T01:49:49Z | - |
dc.date.available | 2011-01-14T01:49:49Z | - |
dc.date.issued | 2008 | - |
dc.identifier.issn | 0730-2312 | - |
dc.identifier.uri | http://repository.ajou.ac.kr/handle/201003/1137 | - |
dc.description.abstract | Sanguinarine is a benzophenanthridine alkaloid derived from the root of Sanguinaria canadensis and other poppy-fumaria species, possessing potent antibacterial, antifungal, and anti-inflammatory activities. In this study, we investigated the underling mechanisms by which sanguinarine induce apoptosis in human breast cancer MDA-231 cells. Treatment of MDA-231 cells with sanguinarine induced remarkable apoptosis accompanying the generation of ROS. Consistently, sanguinarine-induced apoptosis was mediated by the increased reproductive cell death. Pretreatment with NAC or GSH attenuated sanguinarine-induced apoptosis, suggesting the involvement of ROS in this cell death. During sanguinarin-induced apoptosis, protein levels of pro-caspase-3, Bcl-2, cIAP2, XIAP, and c-FLIPs were reduced. Sanguinarine-mediated apoptosis was substantially blocked by ectopic expression of Bcl-2 and cFLIPs. Additionally, we found that sub-lethal doses of sanguinarine remarkably sensitized breast cancer cells to TRAIL-mediated apoptosis, but the cell death induced by sanguinarine and TRAIL in combination was not blocked by overexpression of Bcl-2 or Akt. Therefore, combinatory treatment of sanguinarine and TRAIL may overcome the resistance of breast cancer cells due to overexpression of Akt or Bcl-2. | - |
dc.format | application/pdf | - |
dc.language.iso | en | - |
dc.subject.MESH | Anti-Infective Agents | - |
dc.subject.MESH | Apoptosis | - |
dc.subject.MESH | Benzophenanthridines | - |
dc.subject.MESH | CASP8 and FADD-Like Apoptosis Regulating Protein | - |
dc.subject.MESH | Cell Line, Tumor | - |
dc.subject.MESH | Down-Regulation | - |
dc.subject.MESH | Gene Expression Regulation, Neoplastic | - |
dc.subject.MESH | Glutathione | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Isoquinolines | - |
dc.subject.MESH | Models, Biological | - |
dc.subject.MESH | Plant Extracts | - |
dc.subject.MESH | Proto-Oncogene Proteins c-akt | - |
dc.subject.MESH | Proto-Oncogene Proteins c-bcl-2 | - |
dc.subject.MESH | Reactive Oxygen Species | - |
dc.subject.MESH | TNF-Related Apoptosis-Inducing Ligand | - |
dc.title | Sanguinarine-induced apoptosis: generation of ROS, down-regulation of Bcl-2, c-FLIP, and synergy with TRAIL. | - |
dc.type | Article | - |
dc.identifier.pmid | 18189268 | - |
dc.contributor.affiliatedAuthor | 최, 경숙 | - |
dc.type.local | Journal Papers | - |
dc.identifier.doi | 10.1002/jcb.21672 | - |
dc.citation.title | Journal of cellular biochemistry | - |
dc.citation.volume | 104 | - |
dc.citation.number | 3 | - |
dc.citation.date | 2008 | - |
dc.citation.startPage | 895 | - |
dc.citation.endPage | 907 | - |
dc.identifier.bibliographicCitation | Journal of cellular biochemistry, 104(3). : 895-907, 2008 | - |
dc.identifier.eissn | 1097-4644 | - |
dc.relation.journalid | J007302312 | - |
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