269 467

Cited 28 times in

Cross talk between P2 purinergic receptors modulates extracellular ATP-mediated interleukin-10 production in rat microglial cells.

DC Field Value Language
dc.contributor.authorSeo, DR-
dc.contributor.authorKim, SY-
dc.contributor.authorKim, KY-
dc.contributor.authorLee, HG-
dc.contributor.authorMoon, JH-
dc.contributor.authorLee, JS-
dc.contributor.authorLee, SH-
dc.contributor.authorKim, SU-
dc.contributor.authorLee, YB-
dc.date.accessioned2011-01-14T02:39:56Z-
dc.date.available2011-01-14T02:39:56Z-
dc.date.issued2008-
dc.identifier.issn1226-3613-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/1144-
dc.description.abstractPreviously we demonstrated that ATP released from LPS-activated microglia induced IL-10 expression in a process involving P2 receptors, in an autocrine fashion. Therefore, in the present study we sought to determine which subtype of P2 receptor was responsible for the modulation of IL-10 expression in ATP-stimulated microglia. We found that the patterns of IL-10 production were dose-dependent (1, 10, 100, 1,000 microM) and bell-shaped. The concentrations of ATP, ATP-gammaS, ADP, and ADP-betaS that showed maximal IL-10 release were 100, 10, 100, and 100 microM respectively. The rank order of agonist potency for IL-10 production was 2'-3'-O-(4-benzoyl)-benzoyl ATP (BzATP)=dATP>2-methylthio-ADP (2-meSADP). On the other hand, 2-methylthio-ATP (2-meSATP), alpha,beta-methylene ATP (alpha,beta-meATP), UTP, and UDP did not induce the release of IL-10 from microglia. Further, we obtained evidence of crosstalk between P2 receptors, in a situation where intracellular Ca(2+) release and/or cAMP-activated PKA were the main contributors to extracellular ATP-(or ADP)-mediated IL-10 expression, and IL-10 production was down-regulated by either MRS2179 (a P2Y(1) antagonist) or 5'-AMPS (a P2Y(11) antagonist), indicating that both the P2Y(1) and P2Y(11) receptors are major receptors involved in IL-10 expression. In addition, we found that inhibition of IL-10 production by high concentrations of ATP-gammaS (100 microM) was restored by TNP-ATP (an antagonist of the P2X(1), P2X(3), and P2X(4) receptors), and that IL-10 production by 2-meSADP was restored by 2meSAMP (a P2Y(12) receptor antagonist) or pertussis toxin (PTX; a Gi protein inhibitor), indicating that the P2X(1), P2X(3), P2X(4)receptor group, or the P2Y(12) receptor, negatively modulate the P2Y(11) receptor or the P2Y(1) receptor, respectively.-
dc.language.isoen-
dc.subject.MESHAdenosine Diphosphate/analogs & derivatives-
dc.subject.MESHAdenosine Diphosphate/pharmacology-
dc.subject.MESHAdenosine Triphosphate/analogs & derivatives-
dc.subject.MESHAdenosine Triphosphate/pharmacology*-
dc.subject.MESHAdenylate Cyclase/antagonists & inhibitors-
dc.subject.MESHAnimals-
dc.subject.MESHCalcium/metabolism-
dc.subject.MESHChelating Agents/pharmacology-
dc.subject.MESHCyclic AMP-Dependent Protein Kinases/antagonists & inhibitors-
dc.subject.MESHEnzyme Inhibitors/pharmacology-
dc.subject.MESHExtracellular Space/drug effects-
dc.subject.MESHExtracellular Space/metabolism*-
dc.subject.MESHGene Expression Regulation/drug effects-
dc.subject.MESHInterleukin-10/biosynthesis*-
dc.subject.MESHMicroglia/drug effects*-
dc.subject.MESHMicroglia/enzymology-
dc.subject.MESHMicroglia/metabolism*-
dc.subject.MESHPurinergic P2 Receptor Agonists-
dc.subject.MESHPurinergic P2 Receptor Antagonists-
dc.subject.MESHRNA, Messenger/genetics-
dc.subject.MESHRNA, Messenger/metabolism-
dc.subject.MESHRats-
dc.subject.MESHRats, Sprague-Dawley-
dc.subject.MESHReceptor Cross-Talk/drug effects*-
dc.subject.MESHReceptors, Purinergic P2/genetics-
dc.subject.MESHReceptors, Purinergic P2/metabolism*-
dc.subject.MESHThionucleotides/pharmacology-
dc.titleCross talk between P2 purinergic receptors modulates extracellular ATP-mediated interleukin-10 production in rat microglial cells.-
dc.typeArticle-
dc.identifier.pmid18305394-
dc.identifier.urlhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2679320/-
dc.contributor.affiliatedAuthor이, 용범-
dc.type.localJournal Papers-
dc.identifier.doi10.3858/emm.2008.40.1.19-
dc.citation.titleExperimental & molecular medicine-
dc.citation.volume40-
dc.citation.number1-
dc.citation.date2008-
dc.citation.startPage19-
dc.citation.endPage26-
dc.identifier.bibliographicCitationExperimental & molecular medicine, 40(1):19-26, 2008-
dc.identifier.eissn2092-6413-
Appears in Collections:
Journal Papers > Research Organization > Institute for Medical Sciences
Files in This Item:
emm-40-19-26.pdfDownload

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse