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Characterization of phototransduction gene knockouts revealed important signaling networks in the light-induced retinal degeneration.

Authors
Krishnan, J; Lee, G; Han, SU; Choi, S
Citation
Journal of biomedicine & biotechnology, 2008327468-327468, 2008
Journal Title
Journal of biomedicine & biotechnology
ISSN
1110-72431110-7251
Abstract
Understanding the molecular pathways mediating neuronal function in retinas can be greatly facilitated by the identification of genes regulated in the retinas of different mutants under various light conditions. We attempted to conduct a gene chip analysis study on the genes regulated during rhodopsin kinase (Rhok-/-) and arrestin (Sag-/-) knockout and double knockouts in mice retina. Hence, mice were exposed to constant illumination of 450 lux or 6,000 lux on dilated pupils for indicated periods. The retinas were removed after the exposure and processed for microarray analysis. Double knockout was associated with immense changes in gene expression regulating a number of apoptosis inducing transcription factors. Subsequently, network analysis revealed that during early exposure the transcription factors, p53, c-MYC, c-FOS, JUN, and, in late phase, NFkappaB, appeared to be essential for the initiation of light-induced retinal rod loss, and some other classical pro- and antipoptotic genes appeared to be significantly important as well.
MeSH terms
AnimalsApoptosis Regulatory Proteins/metabolism*Arrestin/geneticsArrestin/metabolism*G-Protein-Coupled Receptor Kinase 1/geneticsG-Protein-Coupled Receptor Kinase 1/metabolism*MiceMice, Inbred C57BLMice, KnockoutRetina/metabolismRetinal Degeneration/metabolism*Retinal Rod Photoreceptor Cells/metabolism*Transcription Factors/metabolism*Vision, Ocular*
DOI
10.1155/2008/327468
PMID
18354737
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Physiology
Journal Papers > School of Medicine / Graduate School of Medicine > Surgery
AJOU Authors
이, 광한, 상욱
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