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Gas chromatographic-mass spectrometric analyses of cholesterol and its precursors in rat plasma as tert-butyldimethylsilyl derivatives.

Authors
Paik, MJ; Yu, J; Hu, MB; Kim, SJ; Kim, KR; Ahn, YH; Choi, S; Lee, G
Citation
Clinica chimica acta: international journal of clinical chemistry, 396(1-2):62-65, 2008
Journal Title
Clinica chimica acta: international journal of clinical chemistry
ISSN
0009-89811873-3492
Abstract
BACKGROUND: Cholesterol and its metabolic precursors occurring in metabolic pathways are important biochemical indicators in pathological conditions. METHODS: A method for the simultaneous determination of cholesterol and its metabolic precursors, such as lanosterol and 7-dehydrocholesterol, in rat plasma is demonstrated. It involves their extraction after saponification, followed by conversion to tert-butyldimethylsilyl (TBDMS) derivatives for analysis by gas chromatography-mass spectrometry (GC-MS) in selected ion monitoring (SIM) mode (GC-SIM-MS). RESULTS: The characteristic fragment ions of [M-57], m/z 443, 483, and 441 permitted the accurate and selective detection of cholesterol and its precursors in rat plasma. The whole procedure of TBDMS derivatization, with subsequent GC-SIM-MS analysis, was linear (r>or=0.9994), reproducible (% relative standard deviation=2.2 to 7.5), and accurate (% relative error=-5.6 to 7.7), with detection limits of 0.02 to 0.07 ng/ml. Recoveries were measured to be ranged from 89.5 to 95.4%. CONCLUSION: The present method was useful for the quantification of cholesterol and its precursors in rat plasma samples of 1 microl.
MeSH terms
AnimalsCholesterol/blood*Gas Chromatography-Mass Spectrometry/methods*MaleMethylationRatsRats, Sprague-DawleySpectrometry, Mass, Electrospray Ionization/methods*
DOI
10.1016/j.cca.2008.06.025
PMID
18657530
Appears in Collections:
Journal Papers > Research Organization > Institute for Neuroregeneration & Stem Cell Research
Journal Papers > School of Medicine / Graduate School of Medicine > Neurosurgery
Journal Papers > School of Medicine / Graduate School of Medicine > Physiology
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