Cited 0 times in
Gene expression profiling of light-induced retinal degeneration in phototransduction gene knockout mice.
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Krishnan, J | - |
dc.contributor.author | Chen, J | - |
dc.contributor.author | Shin, KJ | - |
dc.contributor.author | Hwang, JI | - |
dc.contributor.author | Han, SU | - |
dc.contributor.author | Lee, G | - |
dc.contributor.author | Choi, S | - |
dc.date.accessioned | 2011-01-14T05:26:33Z | - |
dc.date.available | 2011-01-14T05:26:33Z | - |
dc.date.issued | 2008 | - |
dc.identifier.issn | 1226-3613 | - |
dc.identifier.uri | http://repository.ajou.ac.kr/handle/201003/1152 | - |
dc.description.abstract | Exposure to light can induce photoreceptor cell death and exacerbate retinal degeneration. In this study, mice with genetic knockout of several genes, including rhodopsin kinase (Rhok-/-), arrestin (Sag-/-), transducin (Gnat1-/-), c-Fos (c-Fos-/-) and arrestin/transducin (Sag-/-/Gnat1-/-), were examined. We measured the expression levels of thousands of genes in order to investigate their roles in phototransduction signaling in light-induced retinal degeneration using DNA microarray technology and then further explored the gene network using pathway analysis tools. Several cascades of gene components were induced or inhibited as a result of corresponding gene knockout under specific light conditions. Transducin deletion blocked the apoptotic signaling induced by exposure to low light conditions, and it did not require c-Fos/AP-1. Deletion of c-Fos blocked the apoptotic signaling induced by exposure to high intensity light. In the present study, we identified many gene transcripts that are essential for the initiation of light-induced rod degeneration and proposed several important networks that are involved in pro- and anti-apoptotic signaling. We also demonstrated the different cascades of gene components that participate in apoptotic signaling under specific light conditions. | - |
dc.language.iso | en | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Apoptosis | - |
dc.subject.MESH | G-Protein-Coupled Receptor Kinase 1 | - |
dc.subject.MESH | GTP-Binding Protein alpha Subunits | - |
dc.subject.MESH | Gene Expression Profiling | - |
dc.subject.MESH | Genes, fos | - |
dc.subject.MESH | Light | - |
dc.subject.MESH | Light Signal Transduction | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Knockout | - |
dc.subject.MESH | Oligonucleotide Array Sequence Analysis | - |
dc.subject.MESH | Retina | - |
dc.subject.MESH | Retinal Degeneration | - |
dc.subject.MESH | Transducin | - |
dc.title | Gene expression profiling of light-induced retinal degeneration in phototransduction gene knockout mice. | - |
dc.type | Article | - |
dc.identifier.pmid | 18985007 | - |
dc.identifier.url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2679354/ | - |
dc.contributor.affiliatedAuthor | 한, 상욱 | - |
dc.contributor.affiliatedAuthor | 이, 광 | - |
dc.type.local | Journal Papers | - |
dc.identifier.doi | 10.3858/emm.2008.40.5.495 | - |
dc.citation.title | Experimental & molecular medicine | - |
dc.citation.volume | 40 | - |
dc.citation.number | 5 | - |
dc.citation.date | 2008 | - |
dc.citation.startPage | 495 | - |
dc.citation.endPage | 504 | - |
dc.identifier.bibliographicCitation | Experimental & molecular medicine, 40(5). : 495-504, 2008 | - |
dc.identifier.eissn | 2092-6413 | - |
dc.relation.journalid | J012263613 | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.