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Mesenchymal stem cells therapy exerts neuroprotection in a progressive animal model of Parkinson's disease.

Authors
Park, HJ; Lee, PH; Bang, OY; Lee, G; Ahn, YH
Citation
Journal of neurochemistry, 107(1):141-151, 2008
Journal Title
Journal of neurochemistry
ISSN
0022-30421471-4159
Abstract
Parkinson's disease is a common progressive neurodegenerative disorder caused by the loss of dopaminergic neurons in the substantia nigra. We investigated whether cell therapy with human mesenchymal stem cells (hMSCs) had a protective effect on progressive dopaminergic neuronal loss in vitro and in vivo. In primary mesencephalic cultures, hMSCs treatment significantly decreased MG-132-induced dopaminergic neuronal loss with a significant reduction of caspase-3 activity. In rats received systemic injection of MG-132, hMSCs treatment in MG-132-treated rats dramatically reduced the decline in the number of tyrosine hydroxylase (TH)-immunoreactive cells, showing an approximately 50% increase in the survival of TH-immunoreactive cells in the substantia nigra compared with the MG-132-treated group. Additionally, hMSC treatment significantly decreased OX-6 immunoreactivity and caspase-3 activity. Histological analysis showed that the number of NuMA-positive cells was 1.7% of total injected hMSCs and 35.7% of these cells were double-stained with NuMA and TH. Adhesive-removal test showed that hMSCs administration in MG-132-treated rats had a tendency to decrease in the mean removal time. This study demonstrates that hMSCs treatment had a protective effect on progressive loss of dopaminergic neurons induced by MG-132 in vitro and in vivo. Complex mechanisms mediated by trophic effects of hMSCs and differentiation of hMSCs into functional TH-immunoreactive neurons may work in the neuroprotective process.
MeSH terms
AnimalsCaspase 3/metabolismCell Communication/physiologyCell CountCell Death/physiologyCell Differentiation/physiologyCell Survival/physiologyCysteine Proteinase Inhibitors/pharmacologyCytoprotection/physiology*Disease Models, AnimalDopamine/metabolismHumansLeupeptins/pharmacologyMaleMesenchymal Stem Cell Transplantation/methods*Mesenchymal Stem Cells/metabolism*Mesenchymal Stem Cells/secretionNerve Growth Factors/metabolismNerve Growth Factors/secretionNeurons/metabolism*Parkinsonian Disorders/metabolismParkinsonian Disorders/physiopathologyParkinsonian Disorders/therapy*RatsRats, Sprague-DawleySubstantia Nigra/metabolism*Substantia Nigra/physiopathologySubstantia Nigra/surgeryTreatment OutcomeTyrosine 3-Monooxygenase/metabolism
DOI
10.1111/j.1471-4159.2008.05589.x
PMID
18665911
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Physiology
Journal Papers > School of Medicine / Graduate School of Medicine > Neurosurgery
AJOU Authors
이, 광안, 영환
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