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N-ethoxycarbonylation combined with (S)-1-phenylethylamidation for enantioseparation of amino acids by achiral gas chromatography and gas chromatography-mass spectrometry.

Authors
Paik, MJ; Lee, J; Kim, KR
Citation
Journal of chromatography. A, 1214(1-2):151-156, 2008
Journal Title
Journal of chromatography. A
ISSN
0021-96731873-3778
Abstract
N-ethoxycarbonylation was combined with (S)-1-phenylethylamidation for enantioseparation of amino acids by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) on achiral capillary columns. The method provided complete enantioseparations of 12 amino acids as diastereomeric N-ethoxycarbonyl/(S)-1-phenylethylamides with exceptional resolutions for proline (R(s) > or = 9.9) and pipecolic acid (R(s) > or = 10.2). GC-MS analysis in selected ion monitoring mode employing standard addition method, facilitated quantitation of D-pipecolic acid in kidney bean (0.95 microg/10 mg) and adzuki bean (0.14 microg/10 mg). The peak area ratios indicated that they had the identical chiral composition at 2.5% for D-pipecolic acid and 97.5% for L-pipecolic acid.
MeSH terms
Amino Acids/analysis*Amino Acids/chemistryChromatography, Gas/methods*Fabaceae/chemistryFormic Acid Esters/metabolismGas Chromatography-Mass Spectrometry/methods*Least-Squares AnalysisPhaseolus/chemistryPhenethylamines/metabolismReproducibility of ResultsSensitivity and SpecificityStereoisomerism
DOI
10.1016/j.chroma.2008.10.068
PMID
18995862
Appears in Collections:
Journal Papers > Research Organization > Institute for Neuroregeneration & Stem Cell Research
AJOU Authors
백만정
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