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Methyltransferase-inhibition interferes with neuronal differentiation of P19 embryonal carcinoma cells.

Authors
Hong, S; Heo, J; Lee, S; Heo, S; Kim, SS; Lee, YD; Kwon, M
Citation
Biochemical and biophysical research communications, 377(3):935-940, 2008
Journal Title
Biochemical and biophysical research communications
ISSN
0006-291X1090-2104
Abstract
We have analyzed the importance of substrate methylation by S-adenosylmethionine-dependent methyltransferases for neuronal differentiation of P19 embryonal carcinoma cells. We show that treatment of cells with methyltransferase inhibitor adenosine dialdehyde (AdOx) interferes with neuronal differentiation. Retinoic acid (RA) and AdOx co-treated cells had a decreased number of neurites and a flattened morphology compared with cells differentiated by RA. Also, the amount of neuronal class III tubulin (Tuj1) decreased from 76% to 9.6% with AdOx-treatment. Gene expression levels of wnt-1, brn-2, neuroD, and mash-1 were also down-regulated by AdOx-treatment. But AdOx-treatment did not up-regulate BMP-4 and GFAP genes. Treatment of RA decreased E-cadherin expression during neuronal differentiation. However, in AdOx/RA co-treated cells, E-cadherin expression was restored to the control level. Also, mRNA expression of N-cadherin decreased with AdOx-treatment. Taken together, these data show that methylation reactions might influence the cell-fate decision and neuronal differentiation of P19 cells.
MeSH terms
Adenosine/analogs & derivativesAdenosine/pharmacologyAnimalsCadherins/biosynthesisCarcinoma, EmbryonalCell Communication/drug effectsCell Communication/geneticsCell Line, TumorEnzyme Inhibitors/pharmacologyGene Expression Regulation, DevelopmentalMesoderm/cytologyMesoderm/enzymologyMethylation/drug effectsMethyltransferases/antagonists & inhibitorsMethyltransferases/metabolism*MiceNeurogenesis*/drug effectsNeurogenesis*/geneticsNeuroglia/cytologyNeuroglia/enzymologyNeurons/cytology*Neurons/enzymology*RNA, Messenger/biosynthesisTranscription, Genetic/drug effects
DOI
10.1016/j.bbrc.2008.10.089
PMID
18957285
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Anatomy
AJOU Authors
김, 성수이, 영돈
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