186 268

Cited 0 times in

Characterization of cathepsin B cysteine protease from Naegleria fowleri excretory-secretory proteins exerting immunomodulatory effects

Authors
이, 진영
Department
대학원 의생명과학과
Degree
Doctor (2015)
Abstract
Naegleria fowleri, a free-living amoeba that is found in diverse environmental

habitats, can cause an acute fulminating hemorrhagic meningoencephalitis, called

primary amoebic meningoencephalitis (PAM) in experimental animals and in

humans. Pathogenicity of N. fowleri may be induced by contact-dependent and

contact-independent mechanisms, and both mechanisms lead to death of host cells.

Several proteins secreted from N. fowleri are likely to be associated with contactindependent

pathogenic mechanism of the amoeba. The excretory and secretory

proteins of N. fowleri (Nf-ESPs) include phospholipase, proteases, peroxiredoxins

and thrombin receptor. However, the precise mechanism induced by Nf-ESPs in

PAM is not fully understood yet. In this study, the cytopathic changes and

inflammatory responses induced by Nf-ESPs were analyzed in BV2 microglial

cells. Several cytokines, particularly IL-1α and TNF- α were highly up-regulated

by Nf-ESPs. Also, activation of P38 and JNK was observed in BV2 cells treated

with Nf-ESPs. The results collectively suggest that Nf-ESPs may play an

important role in N. fowleri mediated-PAM through induction of inflammatory

response in microglial cells and activation of MAPKs signal pathway.
Keywords
Naegleria fowleriexcretory/secretory proteinscytokineMAPKmicroglial cell파울러자유아메바시스테인 단백분해효소배설-분비 단백질Cathepsin B 단백질Cathepsin B-like 단백질
Appears in Collections:
Theses > Graduate School of Biomedical Sciences > Doctor
AJOU Authors
이, 진영
Full Text Link
Files in This Item:
000000019230.pdfDownload
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse