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The role of ATM responding to DNA damage induced by Xrcc1 deficiency during oligodendrocyte genesis

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dc.contributor.author최, 인서-
dc.date.accessioned2015-10-26T01:13:33Z-
dc.date.available2015-10-26T01:13:33Z-
dc.date.issued2015-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/11815-
dc.description.abstractAtaxia telangiectasia (A-T) is a prime example of hereditary disease about DNA damage signaling and repair protein deficiency. A-T is an autosomal recessive neurodegenerative disorder associated the defective ATM (Ataxia telangiectasia mutated) gene that is estimated to affect 1 in 40,000-300,000 people. The clinical features manifested in A-T patients are multisystem symptoms, including cerebellar atrophy, ataxia, abnormal eyes movement, telangiectatic blood vessel, immunodeficiency and hyper-radiosensitivity. Currently there is no cure and no way to slow neuropathological progression of the A-T patients. Because, different from other animal models to mimic diseases with DNA repair protein deficiency, ATM-/- mice do not recapitulate the phenotypes of A-T patients, making it difficult to study the mechanism of neurological phenotypes of this disease in mice.

To make the proper mice model for A-T, we generated Xrcc1 and Atm double knockout mice (hereafter referred to as Xrcc1nes-cre; Atm-/-) to determine the function of DNA damage sensor kinase Atm upon persistent DNA damage condition by Xrcc1 inactivation during brain development. Xrcc1 is a scaffolding protein that is recruited to DNA single strand break site with several interacting proteins such as PARP1, DNA polymeraseβ and Ligase 3. Loss of Xrcc1 led to the persistence of endogenous DNA strand breaks. Xrcc1nes-cre; Atm-/- mouse recapitulates the neurological phenotypes of A-T patients including cerebellar ataxia, cerebellar atrophy, defect of Purkinje cell and dysmyelination in the brain. In the case of dysmyelination, this is a subset of white matter disorders characterized by differentiation defect of oligodendrocytes, also known as leukodystrophy.

We found that neurons and astrocytes in the Xrcc1nes-cre; Atm-/- brain underwent normal differentiation and maturation in vivo and in vitro. But, fully mature oligodendrocyte markers, MBP, PLP and CNPase were downregulated in the Xrcc1nes-cre; Atm-/- mouse cortex and cerebellum. But, the preceding state of fully mature oligodendrocytes, oligodendrocyte progenitor cells (OPC) were intact without any defect such as cell cycle progression and cell death in vivo and in vitro. This outcome was affected by the low protein levels of the oligodendrocyte transcription factor, Olig1. Taken together, our data demonstrate Atm is critical for the proper development of cerebellum and neural cell differentiation, especially oligodendrocytes.
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dc.description.tableofcontentsI. INTRODUCTION

II. MATERIAL AND METHODS

A. Generation of Xrcc1 and Atm knockout animal model

B. Isolation and differentiation of primary mouse neural stem cell

C. Determination of cell number in proliferation

D. Antibodies for histology and western blot

E. Immunohistochemistry

F. TUNEL assay

G. Protein extraction (nuclear/cytosol) and western blot analysis

H. RNA extraction and quantitative real time PCR

III. RESULTS

1. Double inactivation of Xrcc1 and Atm in the central nervous system

2. Loss of Xrcc1 and Atm results in DNA repair deficiency in the brain

3. Cerebellar interneurons are not rescued in the Xrcc1nes-cre; Atm-/- brain

4. Purkinje cells are susceptible to Xrcc1 and Atm loss

5. Xrcc1 and Atm required for oligodendrocyte differentiation

6. Oligodendrocyte progenitor cell formation in the Xrcc1nes-cre; Atm-/- brain

7. Oligodendrocyte specific transcription factor Olig1 is affected by Atm signaling

IV. DISCUSSION

V. REFFERENCES

-국문요약-
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dc.language.isoen-
dc.titleThe role of ATM responding to DNA damage induced by Xrcc1 deficiency during oligodendrocyte genesis-
dc.title.alternativeXrcc1의 결함에 의한 DNA 손상발생시 희소돌기아교세포의 형성과정동안 ATM의 역할-
dc.typeThesis-
dc.identifier.urlhttp://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000020415-
dc.subject.keywordDNA damage-
dc.subject.keywordAtaxia telangiectasia-
dc.subject.keywordATM-
dc.subject.keywordNeurodegeneration-
dc.subject.keywordOligodendrocyte-
dc.description.degreeMaster-
dc.contributor.department대학원 의생명과학과-
dc.contributor.affiliatedAuthor최, 인서-
dc.date.awarded2015-
dc.type.localTheses-
dc.citation.date2015-
Appears in Collections:
Theses > Graduate School of Biomedical Sciences > Master
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