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CDDO-ME induces apoptosis in breast cancer cells via Ca2+ influx-mediated dilation of endoplasmic reticulum and c-FLIP downregulation

DC Field Value Language
dc.contributor.author정, 수아-
dc.date.accessioned2015-10-28-
dc.date.available2015-10-28-
dc.date.issued2015-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/11827-
dc.description.tableofcontentsI.INTRODUCTION 1

II.MATERIALS AND METHODS 6

A.Chemicals and antibodies 6

B.Cell culture of various cancer cell lines 7

C.Measurement of cell viability 7

D.Western blotting 7

E.Immunocytochemistry 8

F.Establishment of the stable cell lines in the fluorescence specifically mitochondria or endoplasmic reticulum 9

G.Measurement of ROS and mitochondrial superoxide anion 9

H.Measurement of cytosolic and mitochondrial Ca² levels 9

I.shRNA-mediated knockdown of proteins 10

J.Transmission electron microscopy 10

K.Reverse transcriptionPCR analysis 11

L.Statistical analysis 12

III. RESULTS 13

1.CDDO-ME demonstrates a potent anti-cancer effect on breast cancer cells 13

2.CDDO-ME induces paraptosis-like cellular vacuolation prior to morphologies features of apoptosis in breast cancer cells 17

3.Ca2+ influx is crucial for CDDO-ME-induced vacuolation and subsequent apoptotic cell death 40

4.Cross-modulation between Ca2+ influx and ROS generation critically contributes to CDDO-ME-induced vacuolation and subsequent apoptosis 51

5.c-FLIPL downregulation plays a critical role in CDDO-ME-induced apoptotic cell death, but not in vacuolation 58

6.Higher increase in intracellular Ca2+ and ROS levels as well as c-FLIP downregulation may contribute to a more potent anti-cancer effect of CDDO-ME, compared to CDDO 69

IV.DISCUSSION 73

V.REFERENCES 79

-국문요약- 90
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dc.formatapplication/pdf-
dc.language.isoen-
dc.titleCDDO-ME induces apoptosis in breast cancer cells via Ca2+ influx-mediated dilation of endoplasmic reticulum and c-FLIP downregulation-
dc.title.alternative악성 유방암 세포에서 CDDO-ME 처리 시 Ca2+ 유입에 매개된 소포체 팽창과 c-FLIP 감소를 통한 세포자살 유도-
dc.typeThesis-
dc.identifier.urlhttp://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000019228-
dc.description.degreeMaster-
dc.contributor.department대학원 의생명과학과-
dc.contributor.affiliatedAuthor정, 수아-
dc.date.awarded2015-
dc.type.localTheses-
dc.citation.date2015-
Appears in Collections:
Theses > Graduate School of Biomedical Sciences > Master
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