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The effect of iron metabolism on palmitate-induced INS-1 cell death

DC Field Value Language
dc.contributor.author정, 익락-
dc.date.accessioned2015-10-28T03:10:47Z-
dc.date.available2015-10-28T03:10:47Z-
dc.date.issued2015-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/11837-
dc.description.tableofcontentsⅠ. INTRODUCTION 1

Ⅱ. MATERIALS AND METHODS 24

A. MATERIALS 24

1. Reagents 24

B. METHODS 25

1. Cell Culture 25

2. Preparation of palmitate 25

3. Isolation of islets 25

4. MTT-bases viability assay 26

5. Measurement of cell death 26

6. Western blot analysis 27

7. RNA extraction and microarray analysis 27

8. Reverse transcriptase-polymerase chain reaction and quantitative PCR 28

9. DNA and RNA Transfection 28

10. Measurement of intracellular labile iron pool (LIP) 29

11. Aconitase activity assay 29

12. Oxygen consumption rate (OCR) 30

13. PA uptake 31

14. Quantitation of insulin 31

15. Immunocytochemistry 32

16. Statistics 32

Ⅲ. RESULTS [Part I] 36

A. Palmitate treatment reduces intracellular iron level in INS-1 beta cell. 36

B. Iron depletion and supplementation in palmitate-induced INS-1 cell death. 41

C. Toxic mediator of iron deficiency. 50

D. Beta-cell function by iron regulation. 60

E. Effect of iron regulation on lipotoxicity in other cells. 61

Ⅲ. RESULTS [Part II] 64

A. Control of PA-induced INS-1 cell death through the TCA cycle intermediate. 64

B. Role of SFA and SFC in various of cell death models. 71

C. The effect of aconitase inhibition on PA-induced lipotoxicity 76

D. SFC altered iron-regulating proteins. 84

E. Changes in intracellular signaling associated with SFC treatment. 88

F. Changes in oxygen consumption rate associated with SFC treatment. 98

G. SFC modulates CD36 translocation and reduces PA uptake. 100

H. SFA and SFC protect against PA-induced islet cell death. 103

Ⅳ. DISCUSSION [Part I] 104

Ⅳ. DISCUSSION [Part II] 110

Ⅴ. CONCLUSION 117

REFERENCES 118

국문요약 135
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dc.formatapplication/pdf-
dc.language.isoen-
dc.titleThe effect of iron metabolism on palmitate-induced INS-1 cell death-
dc.title.alternative고농도 지방산에 의한 INS-1 세포사에 철 대사가 미치는 영향-
dc.typeThesis-
dc.identifier.urlhttp://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000020143-
dc.subject.keywordApoptosis-
dc.subject.keywordEndoplasmic reticulum(ER) stress-
dc.subject.keywordLipotoxicity-
dc.subject.keywordIron-
dc.subject.keywordINS-1 Cell-
dc.subject.keywordPalmitate-
dc.subject.keywordTransferrin receptor-
dc.subject.keywordSoduim fluorocitrate-
dc.description.degreeDoctor-
dc.contributor.department대학원 의생명과학과-
dc.contributor.affiliatedAuthor정, 익락-
dc.date.awarded2015-
dc.type.localTheses-
dc.citation.date2015-
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