280 553

Cited 6 times in

The alpha subunit of Go modulates cell proliferation and differentiation through interactions with Necdin.

Authors
Ju, H | Lee, S | Kang, S | Kim, SS  | Ghil, S
Citation
Cell communication and signaling : CCS, 12. : 39-39, 2014
Journal Title
Cell communication and signaling : CCS
ISSN
1478-811X
Abstract
BACKGROUND:



Heterotrimeric GTP-binding proteins (G-proteins) play an important role in mediating signal transduction generated by neurotransmitters or hormones. Go, a member of the Gi/Go subfamily, is the most abundant G-protein found in the brain. Recently, the alpha subunit of Go (Gαo) was characterized as an inducer of neuronal differentiation. However, its underlying molecular mechanisms have remained unclear to date, since the downstream effectors of Gαo are ambiguous.



RESULTS:



A neurally differentiated embryonal carcinoma-derived protein (Necdin) was isolated as an interacting partner for Gαo from a mouse brain cDNA library using yeast two-hybrid screening. Interactions between the proteins were confirmed with several affinity binding assays, both in vitro and in vivo. Necdin interacted directly and preferentially with activated Gαo, compared to wild-type protein. Interestingly, Gαo did not interact with Gαi, despite high sequence homology between the two proteins. We subsequently analyzed whether Gαo modulates the cellular activities of Necdin. Notably, expression of Gαo significantly augmented Necdin-mediated cellular responses, such as proliferation and differentiation. Moreover, activation of type 1 cannabinoid receptor (CB1R), a Gi/oα-coupled receptor, augmented cell growth suppression, which was mediated by Gαo and Necdin in U87MG cells containing CB1R, Gαo, and Necdin as normal components.



CONCLUSIONS:



These results collectively suggest that Necdin is a candidate downstream effector for Gαo. Our findings provide novel insights into the cellular roles of Gαo and its coupled receptor.
MeSH

DOI
10.1186/s12964-014-0039-9
PMID
25012566
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Anatomy
Ajou Authors
김, 성수
Full Text Link
Files in This Item:
25012566.pdfDownload
Export

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse