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Ratiometric two-photon fluorescent probe for quantitative detection of β-galactosidase activity in senescent cells.

Authors
Lee, HW | Heo, CH | Sen, D | Byun, HO  | Kwak, IH | Yoon, G  | Kim, HM
Citation
Analytical chemistry, 86(20). : 10001-10005, 2014
Journal Title
Analytical chemistry
ISSN
0003-27001520-6882
Abstract
We reported a ratiometric two-photon fluorescent probe (SG1) for β-galactosidase (β-gal) and its application to quantitative detection of β-gal activity during cellular senescence in live cells and in aged tissues. This probe is characterized by a significant two-photon excited fluorescence, a marked blue-to-yellow emission color change in response to β-gal, easy loading, insensitivity to pH and reactive oxygen species (ROS), high photostability, and low cytotoxicity. In addition, we show that SG1 labeling is an effective tool for quantitative detection of senescence-associated β-gal activity at the subcellular level in situ. This finding demonstrates that SG1 will find useful applications in biomedical research, including studies of cell aging.
MeSH
DOI
10.1021/ac5031013
PMID
25293957
Appears in Collections:
Journal Papers > Research Organization > Inflamm-aging Translational Research Center
Journal Papers > School of Medicine / Graduate School of Medicine > Biochemistry & Molecular Biology
Ajou Authors
변, 해옥  |  윤, 계순
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