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Accumulation of cytolytic CD8+ T cells in B16-melanoma and proliferation of mature T cells in TIS21-knockout mice after T cell receptor stimulation.

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dc.contributor.authorRyu, MS-
dc.contributor.authorWoo, MY-
dc.contributor.authorKwon, D-
dc.contributor.authorHong, AE-
dc.contributor.authorSong, KY-
dc.contributor.authorPark, S-
dc.contributor.authorLim, IK-
dc.date.accessioned2015-11-19T05:45:30Z-
dc.date.available2015-11-19T05:45:30Z-
dc.date.issued2014-
dc.identifier.issn0014-4827-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/11995-
dc.description.abstractIn vivo and in vitro effects of TIS21 gene on the mature T cell activation and antitumor activities were explored by employing MO5 melanoma orthograft and splenocytes isolated from the TIS21-knockout (KO)(2) mice. Proliferation and survival of mature T cells were significantly increased in the KO than the wild type (WT3)e cells, indicating that TIS21 inhibits the rate of mature T cell proliferation and its survival. In MO5 melanoma orthograft model, the KO mice recruited much more CD8(+) T cells into the tumors at around day 14 after tumor cell injection along with reduced tumor volumes compared with the WT. The increased frequency of granzyme B+ CD8+ T cells in splenocytes of the KO mice compared with the WT may account for antitumor-immunity of TIS21 gene in the melanoma orthograft. In contrast, reduced frequencies of CD107a+ CD8+ T cells in the splenocytes of KO mice may affect the loss of CD8+ T cell infiltration in the orthograft at around day 19. These results indicate that TIS21 exhibits antiproliferative and proapoptotic effects in mature T cells, and differentially affects the frequencies of granzyme B+ CD8+ T-cells and CD107a+ CD8+ T-cells, thus transiently regulating in vivo anti-tumor immunity.-
dc.language.isoen-
dc.subject.MESHAnimals-
dc.subject.MESHApoptosis-
dc.subject.MESHBlotting, Western-
dc.subject.MESHCD8-Positive T-Lymphocytes-
dc.subject.MESHCell Proliferation-
dc.subject.MESHEnzyme-Linked Immunosorbent Assay-
dc.subject.MESHFemale-
dc.subject.MESHFlow Cytometry-
dc.subject.MESHGranzymes-
dc.subject.MESHImmediate-Early Proteins-
dc.subject.MESHImmunoenzyme Techniques-
dc.subject.MESHLymphocyte Activation-
dc.subject.MESHMale-
dc.subject.MESHMelanoma, Experimental-
dc.subject.MESHMice-
dc.subject.MESHMice, Inbred C57BL-
dc.subject.MESHMice, Knockout-
dc.subject.MESHRNA, Messenger-
dc.subject.MESHReal-Time Polymerase Chain Reaction-
dc.subject.MESHReceptors, Antigen, T-Cell-
dc.subject.MESHSpleen-
dc.subject.MESHT-Lymphocytes, Cytotoxic-
dc.subject.MESHTumor Cells, Cultured-
dc.subject.MESHTumor Suppressor Proteins-
dc.titleAccumulation of cytolytic CD8+ T cells in B16-melanoma and proliferation of mature T cells in TIS21-knockout mice after T cell receptor stimulation.-
dc.typeArticle-
dc.identifier.pmid25088256-
dc.contributor.affiliatedAuthor유, 민숙-
dc.contributor.affiliatedAuthor박, 선-
dc.contributor.affiliatedAuthor임, 인경-
dc.type.localJournal Papers-
dc.identifier.doi10.1016/j.yexcr.2014.07.028-
dc.citation.titleExperimental cell research-
dc.citation.volume327-
dc.citation.number2-
dc.citation.date2014-
dc.citation.startPage209-
dc.citation.endPage221-
dc.identifier.bibliographicCitationExperimental cell research, 327(2). : 209-221, 2014-
dc.identifier.eissn1090-2422-
dc.relation.journalidJ000144827-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Allergy
Journal Papers > School of Medicine / Graduate School of Medicine > Microbiology
Journal Papers > School of Medicine / Graduate School of Medicine > Biochemistry & Molecular Biology
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