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Attenuation of noise-induced hearing loss using methylene blue.

Authors
Park, JS; Jou, I; Park, SM
Citation
Cell death & disease, 5:e1200-e1200, 2014
Journal Title
Cell death & disease
ISSN
2041-4889
Abstract
The overproduction of reactive oxygen species (ROS) and reactive nitrogen species (RNS) has been known to contribute to the pathogenesis of noise-induced hearing loss. In this study, we discovered that in BALB/c mice pretreatment with methylene blue (MB) for 4 consecutive days significantly protected against cochlear injury by intense broad-band noise for 3 h. It decreased both compound threshold shift and permanent threshold shift and, further, reduced outer hair cell death in the cochlea. MB also reduced ROS and RNS formation after noise exposure. Furthermore, it protected against rotenone- and antimycin A-induced cell death and also reversed ATP generation in the in vitro UB-OC1 cell system. Likewise, MB effectively attenuated the noise-induced impairment of complex IV activity in the cochlea. In addition, it increased the neurotrophin-3 (NT-3) level, which could affect the synaptic connections between hair cells and spiral ganglion neurons in the noise-exposed cochlea, and also promoted the conservation of both efferent and afferent nerve terminals on the outer and inner hair cells. These findings suggest that the amelioration of impaired mitochondrial electron transport and the potentiation of NT-3 expression by treatment with MB have a significant therapeutic value in preventing ROS-mediated sensorineural hearing loss.
MeSH terms
Acoustic StimulationAnimalsAuditory Threshold/drug effectsCell Respiration/drug effectsCell Survival/drug effectsCochlea/drug effectsCochlea/pathologyCochlea/physiopathologyCochlea/ultrastructureElectron Transport/drug effectsEvoked Potentials, Auditory, Brain Stem/drug effectsGuanylate Kinase/metabolismHair Cells, Auditory, Inner/drug effectsHair Cells, Auditory, Inner/pathologyHair Cells, Auditory, Inner/ultrastructureHair Cells, Auditory, Outer/drug effectsHair Cells, Auditory, Outer/metabolismHair Cells, Auditory, Outer/pathologyHearing Loss, Noise-Induced/drug therapyHearing Loss, Noise-Induced/pathologyHearing Loss, Noise-Induced/physiopathologyImmunohistochemistryMaleMembrane Proteins/metabolismMethylene Blue/administration & dosageMethylene Blue/pharmacologyMethylene Blue/therapeutic useMice, Inbred BALB CMitochondria/drug effectsMitochondria/metabolismNeurotrophin 3/metabolismReactive Nitrogen Species/metabolismReactive Oxygen Species/metabolismSynapses/drug effectsSynapses/metabolismUp-Regulation/drug effects
DOI
10.1038/cddis.2014.170
PMID
24763057
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Otolaryngology
Journal Papers > School of Medicine / Graduate School of Medicine > Pharmacology
AJOU Authors
박, 정섭주, 일로박, 상면
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