23 259

Cited 4 times in

Hepatocyte growth factor at S phase induces G2 delay through sustained ERK activation.

Authors
Park, YY; Nam, HJ; Lee, JH
Citation
Biochemical and biophysical research communications, 356(1):300-305, 2007
Journal Title
Biochemical and biophysical research communications
ISSN
0006-291X1090-2104
Abstract
The effect of growth factors on the cell cycle progression, except G1/S transition, is poorly understood. Herein, we examined the effect of hepatocyte growth factor (HGF) treated at S phase on the cell cycle progression of HeLa cells. Interestingly, the treatment resulted in G2 delay, evidenced by flow cytometric and mitotic index analyses. The delay corresponded with the delay of degradation of cyclin A and cyclin B, and the delay of decrease of Cdk1/cyclin B and Cdk2/cyclin A kinase activities. As for the signaling responsible, sustained activation of ERK, but neither of p38MAPK nor of JNK, was observed after HGF treatment at S phase. Furthermore, U0126, an inhibitor of MEK1, and DN-MEK partially abrogated the G2 delay, indicating that activation of MEK-ERK pathway is involved. Taken together, HGF treatment of HeLa cells at S phase induces G2 delay partially through sustained activation of ERK signaling.
MeSH terms
Blotting, WesternButadienes/pharmacologyEnzyme Activation/drug effectsEnzyme Inhibitors/pharmacologyExtracellular Signal-Regulated MAP Kinases/metabolism*Flow CytometryG2 Phase/drug effects*Hela CellsHepatocyte Growth Factor/pharmacology*HumansImmunoprecipitationMitotic IndexNitriles/pharmacologyS Phase/drug effects*Signal Transduction/drug effectsTime Factors
DOI
10.1016/j.bbrc.2007.02.123
PMID
17349975
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Biochemistry & Molecular Biology
AJOU Authors
이재호
Full Text Link
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse