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The role of classical and alternative macrophages in the immunopathogenesis of herpes simplex virus-induced inflammation in a mouse model.

DC Field Value Language
dc.contributor.authorAnower, AK-
dc.contributor.authorShim, JA-
dc.contributor.authorChoi, B-
dc.contributor.authorKwon, HJ-
dc.contributor.authorSohn, S-
dc.date.accessioned2015-12-01T05:27:02Z-
dc.date.available2015-12-01T05:27:02Z-
dc.date.issued2014-
dc.identifier.issn0923-1811-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/12161-
dc.description.abstractBACKGROUND:



The exact mechanism of the inflammatory changes occurring during the development of Behçet's disease (BD) remains unclear.



OBJECTIVE:



We investigated the role of classical (M1) and alternative (M2) activation of macrophages in a herpes simplex virus (HSV)-induced BD mouse model.



METHODS:



The classical vs. alternative activated macrophage ratio (M1/M2 ratio) was calculated by analyzing the surface markers CD16/32 and CD23 as M1 and M2 markers, respectively, by flow cytometry. mRNA expression of interferon (IFN)-γ and interleukin (IL)-6 as M1 and arginase-1, FIZZ-1, and MHC-II as M2 markers were analyzed by reverse transcription-polymerase chain reaction. Cytokine levels were assessed by enzyme-linked immunosorbent assay.



RESULTS:



The M1 phenotype was upregulated in BD mice, and an increased M1/M2 ratio was observed compared to that in asymptomatic BD normal and normal healthy mice. Recombinant (r)IFN-γ significantly increased the M1/M2 ratio (1.74±0.42) compared with that of rIL-4 (0.83±0.20). BD mice treated with rIL-4 showed a decreased M1/M2 ratio (1.2±0.3) compared to that of the rIFN-γ- (2.1±2.3) treated group and also showed ameliorated BD symptoms accompanied by downregulation of IL-17 and IL-6 and up-regulation of IL-4.



CONCLUSION:



Therefore, modulation of macrophage phenotypes could be an effective therapeutic approach for treating BD in the future.
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dc.language.isoen-
dc.subject.MESHAnimals-
dc.subject.MESHBehcet Syndrome-
dc.subject.MESHDisease Models, Animal-
dc.subject.MESHInflammation-
dc.subject.MESHInterferon-gamma-
dc.subject.MESHInterleukin-4-
dc.subject.MESHInterleukin-6-
dc.subject.MESHMacrophages-
dc.subject.MESHMale-
dc.subject.MESHMice-
dc.subject.MESHMice, Inbred ICR-
dc.subject.MESHPentoxifylline-
dc.subject.MESHPhenotype-
dc.subject.MESHSimplexvirus-
dc.titleThe role of classical and alternative macrophages in the immunopathogenesis of herpes simplex virus-induced inflammation in a mouse model.-
dc.typeArticle-
dc.identifier.pmid24280370-
dc.identifier.urlhttps://www.ncbi.nlm.nih.gov/pubmed/24280370-
dc.contributor.affiliatedAuthor손, 성향-
dc.type.localJournal Papers-
dc.identifier.doi10.1016/j.jdermsci.2013.11.001-
dc.citation.titleJournal of dermatological science-
dc.citation.volume73-
dc.citation.number3-
dc.citation.date2014-
dc.citation.startPage198-
dc.citation.endPage208-
dc.identifier.bibliographicCitationJournal of dermatological science, 73(3). : 198-208, 2014-
dc.identifier.eissn1873-569X-
dc.relation.journalidJ009231811-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Microbiology
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