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Molecular cloning and characterization of a cytosolic heat shock protein 70 from Naegleria fowleri.

Authors
Song, KJ; Song, KH; Na, BK; Kim, JH; Kwon, D; Park, S; Pak, JH; Im, KI; Shin, HJ
Citation
Parasitology research, 100(5):1083-1089, 2007
Journal Title
Parasitology research
ISSN
0932-01131432-1955
Abstract
A gene encoding a cytosolic heat shock protein 70 from pathogenic Naegleria fowleri (Nf-cHSP70) was identified. The Nf-cHSP70 was 2,062 bp in length with an open reading frame of 1,980 bp encoding 659 amino acid residues. The deduced amino acid sequence of the gene shared high sequence identities with HSP70s from other parasitic organisms and mammals. The characteristic domains, including N-terminal ATPase domain, calmodulin-binding domain, and EE(D)VD motif, found in HSP70s were also well conserved in this gene. The recombinant Nf-cHSP70 protein showed strong antigenicity against the sera from mice experimentally infected with N. fowleri. Immunofluorescence assay showed that Nf-cHSP70 localized in cytosol of the parasite. The results from semi-quantitative RT-PCR and Western blot analyses demonstrated the expression levels of gene transcripts, and its products were significantly increased at high temperature (42 degrees C). The definitive biological roles of Nf-cHSP70 are not clear, but it may protect the parasite under environmental changes especially high temperature.
MeSH terms
Amino Acid Motifs/geneticsAmino Acid SequenceAnimalsCloning, MolecularConserved SequenceCytosol/chemistryDNA, Protozoan/chemistryDNA, Protozoan/geneticsGene Expression RegulationHSP70 Heat-Shock Proteins/genetics*Microscopy, FluorescenceMolecular Sequence DataNaegleria fowleri/genetics*Open Reading FramesProtein Structure, Tertiary/geneticsProtozoan Proteins/genetics*RNA, Messenger/biosynthesisRNA, Messenger/geneticsRNA, Protozoan/biosynthesisRNA, Protozoan/geneticsReverse Transcriptase Polymerase Chain ReactionSequence Analysis, DNASequence Homology, Amino AcidTemperature
DOI
10.1007/s00436-006-0404-8
PMID
17252278
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Microbiology
AJOU Authors
송, 경주권, 대호박, 선신, 호준
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