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Serum specific IgG response to toluene diisocyanate-tissue transglutaminase conjugate in toluene diisocyanate-induced occupational asthmatics.

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dc.contributor.authorPham, le D-
dc.contributor.authorKim, MA-
dc.contributor.authorYoon, MG-
dc.contributor.authorLee, SI-
dc.contributor.authorShin, YS-
dc.contributor.authorPark, HS-
dc.date.accessioned2016-09-30T07:36:53Z-
dc.date.available2016-09-30T07:36:53Z-
dc.date.issued2014-
dc.identifier.issn1081-1206-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/12585-
dc.description.abstractBACKGROUND: Tissue transglutaminase (tTG) is a post-translational modifying

enzyme located in airway epithelial cells. A potential contribution of serum

specific IgG (sIgG) to tTG in airway inflammation of toluene diisocyanate

(TDI)-induced occupational asthma (OA) has been suggested. OBJECTIVE: To prepare

a TDI-tTG conjugate and detect serum specific antibodies in sera of patients with

TDI-OA to understand this mechanism. METHODS: Ninety-nine patients with TDI-OA,

76 asymptomatic exposed controls, 208 patients with non-OA, and 74 unexposed

controls were enrolled for this study. The TDI-tTG conjugate was prepared and

confirmed by a native gel. Serum sIgG and/or sIgE antibodies to tTG, TDI-tTG, TDI

conjugated to human serum albumin, cytokeratin 19, and serum cytokine levels,

such as interleukin-8, transforming growth factor-beta1, and tissue inhibitor of

metalloproteinase-1, were measured by enzyme-linked immunosorbent assay. The

level of interleukin-8 produced from airway epithelial cells (A549) treated with

tTG was evaluated to investigate the inflammatory effect of tTG and TDI-tTG.

RESULTS: In the TDI-OA group, the prevalence of serum sIgG to TDI-tTG (17.2%) was

higher than that of sIgG to tTG (11.1%), which were significantly higher than

those of the 3 control groups (P < .05 for all groups). TDI-exposed subjects with

high levels of serum sIgG to TDI-tTG had a high prevalence of sIgG to cytokeratin

19 and higher serum levels of transforming growth factor-beta1 and tissue

inhibitor of metalloproteinase-1. The tTG and TDI-tTG dose-dependently increased

interleukin-8 production from A549 cells. CONCLUSION: These findings suggest that

TDI exposure in the workplace binds to tTG to form a conjugate that can induce

serum sIgG antibody production, airway inflammation, and airway remodeling in

patients with TDI-OA.
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dc.language.isoen-
dc.subject.MESHAdult-
dc.subject.MESHAirway Remodeling-
dc.subject.MESHAsthma, Occupational-
dc.subject.MESHCase-Control Studies-
dc.subject.MESHDose-Response Relationship, Immunologic-
dc.subject.MESHFemale-
dc.subject.MESHHumans-
dc.subject.MESHImmunoglobulin E-
dc.subject.MESHImmunoglobulin G-
dc.subject.MESHInterleukin-8-
dc.subject.MESHKeratin-19-
dc.subject.MESHMale-
dc.subject.MESHMiddle Aged-
dc.subject.MESHSerum Albumin-
dc.subject.MESHTissue Inhibitor of Metalloproteinase-1-
dc.subject.MESHToluene 2,4-Diisocyanate-
dc.subject.MESHTransforming Growth Factor beta1-
dc.subject.MESHTransglutaminases-
dc.titleSerum specific IgG response to toluene diisocyanate-tissue transglutaminase conjugate in toluene diisocyanate-induced occupational asthmatics.-
dc.typeArticle-
dc.identifier.pmid24950844-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S1081120614002956-
dc.contributor.affiliatedAuthor신, 유섭-
dc.contributor.affiliatedAuthor박, 해심-
dc.type.localJournal Papers-
dc.identifier.doi10.1016/j.anai.2014.04.022-
dc.citation.titleAnnals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology-
dc.citation.volume113-
dc.citation.number1-
dc.citation.date2014-
dc.citation.startPage48-
dc.citation.endPage54-
dc.identifier.bibliographicCitationAnnals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology, 113(1). : 48-54, 2014-
dc.identifier.eissn1534-4436-
dc.relation.journalidJ010811206-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Allergy
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