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Antifibrotic effect of pirfenidone on human pterygium fibroblasts.

DC Field Value Language
dc.contributor.authorLee, K-
dc.contributor.authorLee, SY-
dc.contributor.authorPark, SY-
dc.contributor.authorYang, H-
dc.date.accessioned2016-11-09T01:33:36Z-
dc.date.available2016-11-09T01:33:36Z-
dc.date.issued2014-
dc.identifier.issn0271-3683-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/12800-
dc.description.abstractOBJECTIVE: The effects of pirfenidone were investigated on cultured human

pterygium fibroblasts (HPFs). METHODS: HPFs were obtained from pterygium surgery

and subjected to primary culture. After treatment with 0.5, 1.0 or 1.5 mg/mL

pirfenidone, MTT and cell migration assays were performed, and procollagen

secretion and TGF-beta expression were measured by Western blotting and

immunofluorescence analysis. RESULTS: Pirfenidone had a significant inhibitory

effect on HPF proliferation, migration and collagen synthesis. There were no

differences between the cells treated with 0.5, 1.0 and 1.5 mg/mL pirfenidone and

the controls in the MTT assay. After 48 h of treatment with 1.0 or 1.5 mg/mL

pirfenidone, TGF-beta expression was significantly decreased. CONCLUSIONS: These

findings demonstrate that pirfenidone inhibits the proliferation, migration and

procollagen secretion of HPFs at nontoxic concentrations by decreasing TGF-beta

expression. Thus, pirfenidone may be considered as a safe adjuvant for pterygium

surgery to prevent recurrence.
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dc.language.isoen-
dc.subject.MESHAnti-Inflammatory Agents, Non-Steroidal-
dc.subject.MESHBlotting, Western-
dc.subject.MESHCell Movement-
dc.subject.MESHCell Proliferation-
dc.subject.MESHCells, Cultured-
dc.subject.MESHFibroblasts-
dc.subject.MESHHumans-
dc.subject.MESHImmunoenzyme Techniques-
dc.subject.MESHProcollagen-
dc.subject.MESHPterygium-
dc.subject.MESHPyridones-
dc.subject.MESHTumor Necrosis Factor-alpha-
dc.titleAntifibrotic effect of pirfenidone on human pterygium fibroblasts.-
dc.typeArticle-
dc.identifier.pmid24400786-
dc.identifier.urlhttp://www.tandfonline.com/doi/full/10.3109/02713683.2013.867063-
dc.contributor.affiliatedAuthor이, 기황-
dc.contributor.affiliatedAuthor양, 홍석-
dc.type.localJournal Papers-
dc.identifier.doi10.3109/02713683.2013.867063-
dc.citation.titleCurrent eye research-
dc.citation.volume39-
dc.citation.number7-
dc.citation.date2014-
dc.citation.startPage680-
dc.citation.endPage685-
dc.identifier.bibliographicCitationCurrent eye research, 39(7). : 680-685, 2014-
dc.identifier.eissn1460-2202-
dc.relation.journalidJ002713683-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Ophthalmology
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