Comparison of the changes of the antigenicities of a hen's egg albumin by a gamma and an electron beam irradiation.
Lee, JW; Seo, JH; Kim, JH; Lee, SY; Byun, MW
Radiation physics and chemistry (Oxford, England : 1993), 76(5):879-885, 2007
Radiation physics and chemistry (Oxford, England : 1993)
The study was conducted to compare the radiation types of a gamma ray and an electron beam for the inhibition and reduction of a food allergy. OVA (2 mg/ml) were irradiated at 3, 5, 7 and 10 kGy. Patterns detected by the SDS-PAGE and an immunoblot showed that the intact OVA band disappeared and that it was dependant upon the radiation doses regardless of the radiation types. Binding abilities of the irradiated OVA against the monoclonal IgG and the egg allergic patients' IgE decreased due to a conformational change of the epitope, but differences from using the two different radiation types were not observed. The results indicate that both the radiation types can be used for an inhibition and a reduction of a food allergy regardless of the radiation types.
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