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Virucidal Effect of Gaseous Chlorine Dioxide on Murine Coronavirus A59

Authors
Kim, Jumi; Shin, Bo-Hye; Song, Kyoung Ju; Kim, Jong Rak; Kim, Kyongmin
Department
Department of Microbiology, Ajou University School of Medicine
Abstract
Chlorine dioxide, ClO2, gas is a powerful disinfectant which is 2.5 and 500,000 times more effective than chlorine-based disinfectants and alcohol, respectively. In this study, we examined whether gaseous chlorine dioxide can inactivate murine coronavirus A59. Chlorine dioxide gas was induced from the Puristic™. Puristic™ is a ready-to-use product, without necessitating ClO2 gas generator on site. The treated concentration of ClO2 was 0.16ppmv/min. We exposed chlorine dioxide gas directly to the aliquots of murine coronavirus A59 to examine the virucidal effect of chlorine dioxide gas. To begin with, we activated chlorine dioxide batch for 11 hrs, then aliquots of viruses were exposed to chlorine dioxide gas for 1, 6, or 12 hrs, respectively and titrated by plaque assays on delayed brain tumor cells. After 6 hrs, the titer of gaseous chlorine dioxide-exposed virus was about 3.5 times lower than that of non-exposed control virus. Compared to 9.5 x 103 plaque forming unit/ml of murine coronavirus A59 after 12 hrs of non-exposure, there were no viable virus after 12 hrs exposure. It should be noted here that gaseous chlorine dioxide can inactivate murine coronavirus A59 in 8% normal bovine serum containing viral inoculum, demonstrating that chlorine dioxide gas may act as a virucide even in high concentrations of organic material. Taken together, the gaseous chlorine dioxide may suggest the new paradigm of disinfection system to block the pathogenic viral infections from abroad and the secondary infections, therefore preventing the drastic socie-economic impact by emerging viral infections, which we have learned from MERS outbreak.
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