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Characterization of a growth-elevated cell line of human bone marrow-derived mesenchymal stem cells by SV40 T-antigen

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dc.contributor.authorLee, KS-
dc.contributor.authorShim, JS-
dc.contributor.authorPaik, MJ-
dc.contributor.authorJoo, WH-
dc.contributor.authorKim, SH-
dc.contributor.authorLee, G-
dc.contributor.authorKim, DW-
dc.date.accessioned2017-03-15T04:18:13Z-
dc.date.available2017-03-15T04:18:13Z-
dc.date.issued2015-
dc.identifier.issn1226-8372-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/13539-
dc.description.abstractHuman bone marrow-derived mesenchymal stem cells (hMSCs) are capable of self-renewal and differentiation into various tissue lineages, attracting attention as tools for use in cell therapy. However, hMSCs have very poor proliferative capacity and a short life span in culture. To overcome this problem, we expressed the T antigen of SV40 in hMSCs because it is known to have the ability to elevate the growth rate of various primary animal cells. We obtained several hMSCs lines (hMSCs-T) known for stable expression of T antigen. Cells expressing T antigen proliferated on the monolayer of hMSCs, forming high density foci. hMSCs-T showed changed morphology and improved growth rate and life span, and demonstrated preservation of the potential for differentiation into osteoblasts. In addition, hMSCs-T did not proliferate in soft agar culture, indicating that the cells did not transform into tumor cells. In order to evaluate metabolic change of amino acids in hMSCs-T compared to primary hMSCs, we investigated altered amino acids (AA) with gas chromatography-mass spectrometry (GC-MS) in selected ion monitoring (SIM) mode (GC-SIM-MS). A total of 14 AAs were positively measured. Results from the Student’s t-test on the hMSCs group mean of the hMSCs-T group showed significantly elevated levels of glycine, proline, pipecolic acid, aspartic acid, lysine and tryptophan, whereas valine, leucine and isoleucine as branched-chain amino acids (BCAAs), and phenylalanine showed a significant decrease. Altered AAs metabolic pattern in the hMSCs-T may explain the disturbance of AA metabolism related to the expression of SV40 T antigen in hMSCs.-
dc.language.isoen-
dc.titleCharacterization of a growth-elevated cell line of human bone marrow-derived mesenchymal stem cells by SV40 T-antigen-
dc.typeArticle-
dc.identifier.urlhttp://link.springer.com/article/10.1007/s12257-014-0730-0-
dc.subject.keywordhMSCs-
dc.subject.keywordSV40 T antigen-
dc.subject.keywordamino acids-
dc.subject.keywordgas chromatography-mass spectrometry-
dc.subject.keywordstable cell line-
dc.contributor.affiliatedAuthor이, 광-
dc.type.localJournal Papers-
dc.identifier.doi10.1007/s12257-014-0730-0-
dc.citation.titleBiotechnology and bioprocess engineering : BBE-
dc.citation.volume20-
dc.citation.number3-
dc.citation.date2015-
dc.citation.startPage498-
dc.citation.endPage505-
dc.identifier.bibliographicCitationBiotechnology and bioprocess engineering : BBE, 20(3). : 498-505, 2015-
dc.identifier.eissn1976-3816-
dc.relation.journalidJ012268372-
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Journal Papers > School of Medicine / Graduate School of Medicine > Physiology
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