Cited 0 times in Scipus Cited Count

Real-Time Intracellular Mg2+ Signaling and Wave Propagation by Subdiffraction-Limit Super-Resolution Microscopy

Authors
Chakkarapani, SK | Lee, S | Lee, G  | Kang, SH
Citation
Bulletin of the Korean Chemical Society, 36(11). : 2589-2595, 2015
Journal Title
Bulletin of the Korean Chemical Society
ISSN
0253-29641229-5949
Abstract
Magnesium ion (Mg2+) is an important intracellular component that plays a fundamental role in many cellular processes. The super-localization of intracellular Mg2+ signaling and wave propagation in a single cell with subdiffraction-limit image resolution is demonstrated for the first time by direct stochastic optical reconstruction microscopy (dSTORM). Mag-fluo-4-AM, an intracellular Mg2+ fluorescent indicator dye, is used for the fluorescence imaging of intracellular Mg2+ in human embryonic kidney 293 (HEK-293) cells as a model. Mag-fluo-4-AM is photoswitched between its fluorescent and dark states and the resulting super-resolution cell image reveals uniform and specific labeling of Mg2+ with thousands of fluorophores per cell and localization precision of 21 ± 1 nm. Furthermore, the real-time dynamics of Mg2+ wave propagation are studied by dSTORM. The sparks and waves show random temporal propagation patterns in nonhomogeneous substructures with 0.2 s time intervals. Thus, dSTORM allows characterization of intracellular processes at sub-nanometre spatial resolution with high temporal resolution.
Keywords

Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Physiology
Ajou Authors
이, 광
Full Text Link
Files in This Item:
There are no files associated with this item.
Export

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse