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Effects of interleukin-13 and montelukast on the expression of zonula occludens-1 in human podocytes.

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dc.contributor.authorPark, SJ-
dc.contributor.authorSaleem, MA-
dc.contributor.authorNam, JA-
dc.contributor.authorHa, TS-
dc.contributor.authorShin, JI-
dc.date.accessioned2017-03-21T06:11:12Z-
dc.date.available2017-03-21T06:11:12Z-
dc.date.issued2015-
dc.identifier.issn0513-5796-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/13610-
dc.description.abstractPURPOSE: The aim of this study was to investigate whether pathologic changes in zonula occludens-1 (ZO-1) are induced by interleukin-13 (IL-13) in the experimental minimal-change nephrotic syndrome (MCNS) model and to determine whether montelukast, a leukotriene receptor antagonist, has an effect on ZO-1 restoration in cultured human podocytes.

MATERIALS AND METHODS: Human podocytes cultured on bovine serum albumin-coated plates were treated with different doses of IL-13 and montelukast and then examined for distribution using confocal microscopy and for ZO-1 protein levels using Western blotting.

RESULTS: ZO-1 was internalized and shown to accumulate in the cytoplasm of human podocytes in an IL-13 dose-dependent manner. High doses (50 and 100 ng/mL) of IL-13 decreased the levels of ZO-1 protein at 12 and 24 h (both p<0.01; n=3), which were significantly reversed by a high dose (0.5 μM) montelukast treatment (p<0.01; n=3).

CONCLUSION: Our results suggest that IL-13 alters the expression of ZO-1, and such alterations in the content and distribution of ZO-1 may be relevant in the pathogenesis of proteinuria in the MCNS model.
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dc.language.isoen-
dc.subject.MESHAcetates-
dc.subject.MESHBlotting, Western-
dc.subject.MESHDose-Response Relationship, Drug-
dc.subject.MESHHumans-
dc.subject.MESHInterleukin-13-
dc.subject.MESHLeukotriene Antagonists-
dc.subject.MESHMicroscopy, Confocal-
dc.subject.MESHPodocytes-
dc.subject.MESHProteinuria-
dc.subject.MESHQuinolines-
dc.subject.MESHTight Junctions-
dc.subject.MESHZonula Occludens-1 Protein-
dc.titleEffects of interleukin-13 and montelukast on the expression of zonula occludens-1 in human podocytes.-
dc.typeArticle-
dc.identifier.pmid25683991-
dc.identifier.urlhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4329354/-
dc.contributor.affiliatedAuthor박, 세진-
dc.type.localJournal Papers-
dc.identifier.doi10.3349/ymj.2015.56.2.426-
dc.citation.titleYonsei medical journal-
dc.citation.volume56-
dc.citation.number2-
dc.citation.date2015-
dc.citation.startPage426-
dc.citation.endPage432-
dc.identifier.bibliographicCitationYonsei medical journal, 56(2). : 426-432, 2015-
dc.identifier.eissn1976-2437-
dc.relation.journalidJ005135796-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Pediatrics & Adolescent Medicine
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