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Biologic response of degenerative living human nucleus pulposus cells to treatment with cytokines.

Authors
Kim, SH  | Kuh, SU | Kim, KN | Park, JY | Cho, KH  | Chin, DK | Kim, KS | Cho, YE
Citation
Yonsei medical journal, 56(1). : 277-286, 2015
Journal Title
Yonsei medical journal
ISSN
0513-57961976-2437
Abstract
PURPOSE: To investigate the molecular responses of various genes and proteins related to disc degeneration upon treatment with cytokines that affect disc-cell proliferation and phenotype in living human intervertebral discs (IVDs). Responsiveness to these cytokines according to the degree of disc degeneration was also evaluated.

MATERIALS AND METHODS: The disc specimens were classified into two groups: group 1 (6 patients) showed mild degeneration of IVDs and group 2 (6 patients) exhibited severe degeneration of IVDs. Gene expression was analyzed after treatment with four cytokines: recombinant human bone morphogenic protein (rhBMP-2), transforming growth factor-β (TGF-β), interleukin-1β (IL-1β), and tumor necrosis factor-α (TNF-α). Molecular responses were assessed after exposure of cells from the IVD specimens to these cytokines via real-time polymerase chain reaction and immunofluorescence staining.

RESULTS: mRNA gene expression was significantly greater for aggrecan, type I collagen, type II collagen, alkaline phosphatase, osteocalcin, and Sox9 in group 1 than mRNA gene expression in group 2, when the samples were not treated with cytokines. Analysis of mRNA levels for these molecules after morphogen treatment revealed significant increases in both groups, which were much higher in group 1 than in group 2. The average number of IVD cells that were immunofluorescence stained positive for alkaline phosphatase increased after treatment with rhBMP-2 and TGF-β in group 1.

CONCLUSION: The biologic responsiveness to treatment of rhBMP-2, TGF-β, TNF-α, and IL-1β in the degenerative living human IVD can be different according to the degree of degeneration of the IVD.
MeSH

DOI
10.3349/ymj.2015.56.1.277
PMID
25510775
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Neurosurgery
Ajou Authors
김, 상현  |  조, 기홍
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