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Effects of MBL2 polymorphisms in patients with diisocyanate-induced occupational asthma.

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dc.contributor.authorKim, SH-
dc.contributor.authorBae, SJ-
dc.contributor.authorPalikhe, S-
dc.contributor.authorYe, YM-
dc.contributor.authorPark, HS-
dc.date.accessioned2017-03-31T10:29:16Z-
dc.date.available2017-03-31T10:29:16Z-
dc.date.issued2015-
dc.identifier.issn1226-3613-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/13736-
dc.description.abstractDiisocyanate (DI) is the most common cause of occupational asthma (OA) in Korea. Mannose-binding lectin (MBL) initiates the lectin complement activation pathway following oxidative stress and plays an important role in the regulation of inflammatory processes. To determine whether there is a genetic association between MBL2 polymorphisms and DI-OA, 99 patients with DI-OA, 99 asymptomatic exposed controls (AECs) and 144 unexposed normal controls were enrolled in this study. Three polymorphisms (-554 G>C, -431A>C and -225 G>C) in the MBL2 promoter were genotyped, and serum MBL levels were determined by enzyme-linked immunosorbent assay. Functional variabilities in the promoter polymorphisms were analyzed by a luciferase reporter assay and electrophoretic mobility shift assay (EMSA). A significantly higher frequency of haplotype (ht) 2 [CAG] was noted in the DI-OA group compared with the AEC group (P=0.044). The patients with DI-OA carrying ht2 [CAG] had significantly lower PC20 methacholine levels (P<0.001) than the non-carriers. The serum MBL levels were significantly higher in the DI-exposed subjects (both the DI-OA patients and AECs) carrying ht1 [GAG] (P=0.028). Luciferase activity was significantly enhanced in ht1 [GAG] compared with ht2 [CAG] in human hepatocarcinoma cells (Hep3B) (P=0.002). The EMSA showed that a -554G probe produced a specific shifted band compared with the -554C probe. These findings suggest that decreased serum MBL levels due to polymorphisms of the MBL2 gene may increase susceptibility to the development of DI-OA in DI-exposed individuals.-
dc.language.isoen-
dc.subject.MESHAdult-
dc.subject.MESHAlleles-
dc.subject.MESHAsthma, Occupational-
dc.subject.MESHCell Line-
dc.subject.MESHFemale-
dc.subject.MESHForced Expiratory Volume-
dc.subject.MESHGene Frequency-
dc.subject.MESHGenotype-
dc.subject.MESHHaplotypes-
dc.subject.MESHHumans-
dc.subject.MESHImmunoglobulin E-
dc.subject.MESHImmunoglobulin G-
dc.subject.MESHIsocyanates-
dc.subject.MESHMale-
dc.subject.MESHMannose-Binding Lectin-
dc.subject.MESHMiddle Aged-
dc.subject.MESHPolymorphism, Genetic-
dc.subject.MESHPolymorphism, Single Nucleotide-
dc.subject.MESHProtein Binding-
dc.subject.MESHTranscriptional Activation-
dc.subject.MESHYoung Adult-
dc.titleEffects of MBL2 polymorphisms in patients with diisocyanate-induced occupational asthma.-
dc.typeArticle-
dc.identifier.pmid25857450-
dc.contributor.affiliatedAuthor김, 승현-
dc.contributor.affiliatedAuthor예, 영민-
dc.contributor.affiliatedAuthor박, 해심-
dc.type.localJournal Papers-
dc.identifier.doi10.1038/emm.2015.10-
dc.citation.titleExperimental & molecular medicine-
dc.citation.volume47-
dc.citation.date2015-
dc.citation.startPagee157-
dc.citation.endPagee157-
dc.identifier.bibliographicCitationExperimental & molecular medicine, 47. : e157-e157, 2015-
dc.identifier.eissn2092-6413-
dc.relation.journalidJ012263613-
Appears in Collections:
Journal Papers > Hospital > Clinical Trial Center
Journal Papers > School of Medicine / Graduate School of Medicine > Allergy
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