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Effects of a continuous electromagnetic field on wound healing in human airway.

Authors
Kim, DH | Kim, HJ  | Gimm, YM | Hong, SP | Jeon, EJ | Park, EY
Citation
The Laryngoscope, 125(7). : 1588-1594, 2015
Journal Title
The Laryngoscope
ISSN
0023-852X1531-4995
Abstract
OBJECTIVES/HYPOTHESIS: The aim of this study was to investigate the effect of a 1.8-GHz continuous electromagnetic field (EMF) on wound healing in a human airway cell-culture system.

STUDY DESIGN: In vitro study using a cell line.

METHODS: Immortalized human bronchial epithelial cells (a BEAS-2B cell line) were exposed to a 1.8-GHz EMF (specific absorption rate = 1.0 W/kg). We evaluated the effect of EMF on the cells using an 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) viability assay, by cell counting, and by using fluorescence-activated cell sorting (FACS) analysis of cell cycle dynamics and apoptosis. Inhibition of migration was tested by a wound-healing assay on scratched cell cultures.

RESULTS: Cell migration in the wound-healing assay was decreased by the EMF treatment compared with controls. The MTT assay and cell counting consistently showed that the EMF used was not cytotoxic and did not inhibit cell proliferation. FACS analysis showed no alterations in the cell-cycle phase distribution or in apoptosis after EMF exposure.

CONCLUSION: EMF can inhibit wound healing in vitro by inhibiting cell migration.
MeSH

DOI
10.1002/lary.25109
PMID
25546313
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Otolaryngology
Ajou Authors
김, 현준
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