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In Vitro Priming of Naı̈ve T-cells with p-Phenylenediamine and Bandrowski's Base.

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dc.contributor.authorGibson, A-
dc.contributor.authorKim, SH-
dc.contributor.authorFaulkner, L-
dc.contributor.authorEvely, J-
dc.contributor.authorPirmohamed, M-
dc.contributor.authorPark, KB-
dc.contributor.authorNaisbitt, DJ-
dc.date.accessioned2017-04-27T04:49:05Z-
dc.date.available2017-04-27T04:49:05Z-
dc.date.issued2015-
dc.identifier.issn0893-228X-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/13964-
dc.description.abstractp-Phenylenediamine (PPD) is a component of hair dye formulations that is associated with T-cell mediated allergic contact dermatitis. Antigen-specific T-cells from allergic contact dermatitis patients are activated with either PPD or the oxidation product, Bandrowski's base. In nonallergic individuals, T-cells that are activated by Bandrowski's base, but not by PPD, are readily detectable. The aim of the current study was to use an in vitro T-cell priming assay to assess the activation of memory and naı̈ve T-cells from healthy donors with PPD and Bandrowski's base, and to compare these responses to those observed from allergic patients. Both PPD and Bandrowski's base-responsive clones were generated from allergic patients. The majority of Bandrowski's base-responsive clones were CD4+ and displayed a lack of PPD reactivity. In contrast, CD4+ and CD8+ clones displaying PPD reactivity were detected. Approximately 25% of these displayed low levels of reactivity to Bandrowski's base. Clones from the allergic patients secreted a range of cytokines including IFN-γ, Il-13, and Il-22. In healthy donors, Bandrowski's base-specific T-cell proliferative responses and cytokine secretion were detected with both naı̈ve and memory T-cells. T-cell clones generated from the Bandrowski's base-responsive cultures responded to Bandrowski's base but not PPD. PPD-specific naı̈ve and memory T-cell responses were not detected from healthy donors. These data show that Bandrowski's base stimulates pre-existing memory T-cells isolated from healthy donors and primes naı̈ve T-cells when the chemical is bound to autologous dendritic cells. Priming naı̈ve T-cells against PPD failed, suggesting an important individual susceptibility factor is missing from the in vitro T-cell priming assay.-
dc.language.isoen-
dc.subject.MESHCell Differentiation-
dc.subject.MESHCell Proliferation-
dc.subject.MESHCells, Cultured-
dc.subject.MESHDermatitis, Allergic Contact-
dc.subject.MESHHair Dyes-
dc.subject.MESHHumans-
dc.subject.MESHInterferon-gamma-
dc.subject.MESHInterleukin-13-
dc.subject.MESHInterleukins-
dc.subject.MESHLeukocytes, Mononuclear-
dc.subject.MESHOxidation-Reduction-
dc.subject.MESHPhenylenediamines-
dc.subject.MESHT-Lymphocytes-
dc.titleIn Vitro Priming of Naı̈ve T-cells with p-Phenylenediamine and Bandrowski's Base.-
dc.typeArticle-
dc.identifier.pmid26355666-
dc.contributor.affiliatedAuthor김, 승현-
dc.type.localJournal Papers-
dc.identifier.doi10.1021/acs.chemrestox.5b00294-
dc.citation.titleChemical research in toxicology-
dc.citation.volume28-
dc.citation.number10-
dc.citation.date2015-
dc.citation.startPage2069-
dc.citation.endPage2077-
dc.identifier.bibliographicCitationChemical research in toxicology, 28(10). : 2069-2077, 2015-
dc.identifier.eissn1520-5010-
dc.relation.journalidJ00893228X-
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