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Three-dimensional assessment of bystander effects of mesenchymal stem cells carrying a cytosine deaminase gene on glioma cells.

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dc.contributor.authorJung, JH-
dc.contributor.authorKim, AA-
dc.contributor.authorChang, DY-
dc.contributor.authorPark, YR-
dc.contributor.authorSuh-Kim, H-
dc.contributor.authorKim, SS-
dc.date.accessioned2017-06-13T06:37:45Z-
dc.date.available2017-06-13T06:37:45Z-
dc.date.issued2015-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/14147-
dc.description.abstractStem cells carrying a suicide gene have emerged as therapeutic candidates for their cytotoxic bystander effects on neighboring cancers, while being non-toxic to other parts of the body. However, traditional cytotoxicity assays are unable to adequately assess the therapeutic effects of bystander cells. Here, we report a method to assess bystander effects of therapeutic stem cells against 3-dimensionally grown glioma cells in real time. U87 glioma cells were stably transduced to express a green fluorescence protein and co-cultivated with mesenchymal stem cells engineered to carry a bacterial cytosine deaminase gene (MSC/CD). Following addition of a 5-fluorocytine (5-FC) prodrug to the co-culture, fluorescence from U87 cells was obtained and analyzed in real time. Notably, the IC50 of 5-FC was higher when U87 cells were grown 3-dimensionally in soft agar medium for 3 weeks, as compared to those grown for one week in two-dimensional monolayer cultures. Additionally, more MSC/CD cells were required to maintain a similar level of efficacy. Since three-dimensional growth of glioma cells under our co-culture condition mimics the long-term expansion of cancer cells in vivo, our method can extend to an in vitro assay system to assess stem cell-mediated anti-cancer effects before advancing into preclinical animal studies.-
dc.language.isoen-
dc.titleThree-dimensional assessment of bystander effects of mesenchymal stem cells carrying a cytosine deaminase gene on glioma cells.-
dc.typeArticle-
dc.identifier.pmid26609476-
dc.identifier.urlhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4633898/-
dc.subject.keyword3D culture-
dc.subject.keywordBystander effect-
dc.subject.keywordgene therapy-
dc.subject.keywordstem cell-
dc.subject.keywordsuicide gene-
dc.contributor.affiliatedAuthor장, 다영-
dc.contributor.affiliatedAuthor서, 해영-
dc.contributor.affiliatedAuthor김, 성수-
dc.type.localJournal Papers-
dc.citation.titleAmerican journal of cancer research-
dc.citation.volume5-
dc.citation.number9-
dc.citation.date2015-
dc.citation.startPage2686-
dc.citation.endPage2696-
dc.identifier.bibliographicCitationAmerican journal of cancer research, 5(9). : 2686-2696, 2015-
dc.identifier.eissn2156-6976-
dc.relation.journalidJ021566976-
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Journal Papers > School of Medicine / Graduate School of Medicine > Anatomy
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