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Enhancing the Therapeutic Efficacy of 2-Deoxyglucose in Breast Cancer Cells Using Cell-cycle Synchronization

Authors
Lee, SJ  | Park, BN  | Roh, JH | An, YS  | Hur, H  | Yoon, JK
Citation
Anticancer research, 36(11). : 5975-5980, 2016
Journal Title
Anticancer research
ISSN
0250-70051791-7530
Abstract
AIM: We assessed the effect of cell-cycle synchronization using the T-type calcium channel inhibitor mibefradil on the anticancer effects of 2-deoxy-D-glucose (2-DG) and glucose metabolism in breast cancer cells. MATERIALS AND METHODS: MDA-MB-231 cells were treated with mibefradil, followed by 2-DG with/without paclitaxel, then cells were assessed for viability. Glucose metabolism was evaluated by (3)H-2-DG uptake, lactate concentration, and membrane glucose transporter 1 expression after mibefradil treatment. RESULTS: Viability was significantly lower in cells receiving the combination therapy of mibefradil and 2-DG relative to 2-DG treatment alone: addition of paclitaxel to the combination therapy further reduced the viability of breast cancer cells. Withdrawal of mibefradil resulted in a significant increase in cellular (3)H-2-DG uptake uptake, a slight accumulation of lactate, and increased membrane glucose transporter 1 expression. CONCLUSION: Mibefradil-induced cell-cycle synchronization enhanced the anticancer activity of 2-DG in breast cancer cells due to an increase in cellular glucose metabolism.
MeSH

DOI
10.21873/anticanres.11185
PMID
27793923
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Nuclear Medicine & Molecular Imaging
Journal Papers > School of Medicine / Graduate School of Medicine > Surgery
Ajou Authors
박, 복남  |  안, 영실  |  윤, 준기  |  이, 수진  |  허, 훈
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