Cited 0 times in Scipus Cited Count

Neurofilament heavy chain expression and neuroplasticity in rat auditory cortex after unilateral and bilateral deafness

DC Field Value Language
dc.contributor.authorPark, MH-
dc.contributor.authorJang, JH-
dc.contributor.authorSong, JJ-
dc.contributor.authorLee, HS-
dc.contributor.authorOh, SH-
dc.date.accessioned2018-05-04T00:25:45Z-
dc.date.available2018-05-04T00:25:45Z-
dc.date.issued2016-
dc.identifier.issn0378-5955-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/15048-
dc.description.abstractDeafness induces many plastic changes in the auditory neural system. For instance, dendritic changes cause synaptic changes in neural cells. SMI-32, a monoclonal antibody reveals auditory areas and recognizes non-phosphorylated epitopes on medium- and high-molecular-weight subunits of neurofilament proteins in cortical pyramidal neuron dendrites. We investigated SMI-32-immunoreactive (-ir) protein levels in the auditory cortices of rats with induced unilateral and bilateral deafness. Adult male Sprague-Dawley rats were divided into unilateral deafness (UD), bilateral deafness (BD), and control groups. Deafness was induced by cochlear ablation. All rats were sacrificed, and the auditory cortices were harvested for real-time quantitative polymerase chain reaction (RT-qPCR) and western blot analyses at 2, 4, 6, and 12 weeks after deafness was induced. Immunohistochemical staining was performed to evaluate the location of SMI-32-ir neurons. Neurofilament heavy chain (NEFH) mRNA expression and SMI-32-ir protein levels were increased in the BD group. In particular, SMI-32-ir protein levels increased significantly 6 and 12 weeks after deafness was induced. In contrast, no significant changes in protein level were detected in the right or left auditory cortices at any time point in the UD group. NEFH mRNA level decreased at 4 weeks after deafness was induced in the UD group, but recovered thereafter. Taken together, BD induced plastic changes in the auditory cortex, whereas UD did not affect the auditory neural system sufficiently to show plastic changes, as measured by neurofilament protein level.-
dc.language.isoen-
dc.subject.MESHAnimals-
dc.subject.MESHAntibodies, Monoclonal-
dc.subject.MESHAuditory Cortex-
dc.subject.MESHDeafness-
dc.subject.MESHDendrites-
dc.subject.MESHHearing Loss-
dc.subject.MESHIntermediate Filaments-
dc.subject.MESHMale-
dc.subject.MESHNeurofilament Proteins-
dc.subject.MESHNeuronal Plasticity-
dc.subject.MESHNeurons-
dc.subject.MESHPyramidal Cells-
dc.subject.MESHRNA, Messenger-
dc.subject.MESHRats-
dc.subject.MESHRats, Sprague-Dawley-
dc.subject.MESHTime Factors-
dc.titleNeurofilament heavy chain expression and neuroplasticity in rat auditory cortex after unilateral and bilateral deafness-
dc.typeArticle-
dc.identifier.pmid27457532-
dc.contributor.affiliatedAuthor장, 정훈-
dc.type.localJournal Papers-
dc.identifier.doi10.1016/j.heares.2016.07.010-
dc.citation.titleHearing research-
dc.citation.volume339-
dc.citation.date2016-
dc.citation.startPage155-
dc.citation.endPage160-
dc.identifier.bibliographicCitationHearing research, 339. : 155-160, 2016-
dc.embargo.liftdate9999-12-31-
dc.embargo.terms9999-12-31-
dc.identifier.eissn1878-5891-
dc.relation.journalidJ003785955-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Otolaryngology
Files in This Item:
There are no files associated with this item.

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse