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The regulation of TIM-3 transcription in T cells involves c-Jun binding but not CpG methylation at the TIM-3 promoter

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dc.contributor.authorYun, SJ-
dc.contributor.authorJun, KJ-
dc.contributor.authorKomori, K-
dc.contributor.authorLee, MJ-
dc.contributor.authorKwon, MH-
dc.contributor.authorChwae, YJ-
dc.contributor.authorKim, K-
dc.contributor.authorShin, HJ-
dc.contributor.authorPark, S-
dc.date.accessioned2018-05-04T00:26:48Z-
dc.date.available2018-05-04T00:26:48Z-
dc.date.issued2016-
dc.identifier.issn0161-5890-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/15187-
dc.description.abstractTim-3 is an immunomodulatory protein that is expressed constitutively on monocytes but is induced in activated T cells. The mechanisms involved in the regulation of TIM-3 transcription are poorly understood. In the present study, we investigated whether methylation of the TIM-3 promoter is involved in regulatingTIM-3 transcription in T cells, and identified a transcription factor that regulates TIM-3 transcription by associating with the TIM-3 minimal promoter region. Pyrosequencing of the TIM-3 promoter up to -1440bp revealed 11 hypermethylated CpG sites and 4 hypomethylated CpG sites in human CD4(+) T cells as well as in CD11b(+) cells. Dimethylation of histone H3 lysine 4 (H3K4), a mark of transcriptional activation, was predominantly found in the proximal TIM-3 promoter -954 to -34bp region, whereas trimethylation of H3K9 and H3K27, which are markers of transcriptional suppression, were mostly observed in the distal promoter -1549 to -1048bp region in human CD4(+) T cells and CD11b(+) cells. However, no change in the methylation status of CpG sites and the histone H3 in the TIM-3 promoter was found during induction of TIM-3 transcription in T cells. Finally, AP-1 involvement in TIM-3 transcription was shown in relation with the TIM-3 minimal promoter -146 to +144bp region. The present study defines the minimal TIM-3 promoter region and demonstrates its interaction with c-Jun during TIM-3 transcription in CD4(+) T cells.-
dc.language.isoen-
dc.subject.MESHCD4-Positive T-Lymphocytes-
dc.subject.MESHCell Separation-
dc.subject.MESHChromatin Immunoprecipitation-
dc.subject.MESHCpG Islands-
dc.subject.MESHDNA Methylation-
dc.subject.MESHGene Expression Regulation-
dc.subject.MESHHepatitis A Virus Cellular Receptor 2-
dc.subject.MESHHistones-
dc.subject.MESHHumans-
dc.subject.MESHJurkat Cells-
dc.subject.MESHLymphocyte Activation-
dc.subject.MESHPolymerase Chain Reaction-
dc.subject.MESHPromoter Regions, Genetic-
dc.subject.MESHProto-Oncogene Proteins c-jun-
dc.subject.MESHTranscription Factor AP-1-
dc.titleThe regulation of TIM-3 transcription in T cells involves c-Jun binding but not CpG methylation at the TIM-3 promoter-
dc.typeArticle-
dc.identifier.pmid27243212-
dc.contributor.affiliatedAuthor권, 명희-
dc.contributor.affiliatedAuthor최, 용준-
dc.contributor.affiliatedAuthor김, 경민-
dc.contributor.affiliatedAuthor신, 호준-
dc.contributor.affiliatedAuthor박, 선-
dc.type.localJournal Papers-
dc.identifier.doi10.1016/j.molimm.2016.05.014-
dc.citation.titleMolecular immunology-
dc.citation.volume75-
dc.citation.date2016-
dc.citation.startPage60-
dc.citation.endPage68-
dc.identifier.bibliographicCitationMolecular immunology, 75. : 60-68, 2016-
dc.embargo.liftdate9999-12-31-
dc.embargo.terms9999-12-31-
dc.identifier.eissn1872-9142-
dc.relation.journalidJ001615890-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Microbiology
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