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A highly sensitive and selective impedimetric aptasensor for interleukin-17 receptor A

Authors
Jo, H | Kim, SK | Youn, H | Lee, H | Lee, K | Jeong, J | Mok, J | Kim, SH  | Park, HS  | Ban, C
Citation
Biosensors & bioelectronics, 81. : 80-86, 2016
Journal Title
Biosensors & bioelectronics
ISSN
0956-56631873-4235
Abstract
Interleukin-17 receptor A (IL-17RA) has been recognized as a valuable biomarker for diverse diseases, including autoimmune diseases. In this work, an electrochemical biosensor with great sensitivity and selectivity toward IL-17RA was fabricated using an IL-17RA aptamer (Kd=14.00nM) for the first time. The aptasensor was manufactured using electrodeposition of gold nanoparticles, and then quantitative detection of IL-17RA was performed based on impedimetry. The developed sensor exhibited a superior analytical performance for IL-17RA with a wide dynamic range of 10-10,000pg/mL in buffer and a detection limit of 2.13pg/mL, which is lower than that of commercially available ELISA kits. In addition, we validated the high specificity of the designed aptasensor to only IL-17RA, which showed good sensitivity even in human serum solution. Furthermore, the detection of the differentiated HL-60 cells expressing IL-17RA was successfully performed. Clinical applicability of the sensor was also demonstrated utilizing neutrophils separated from asthma patients. It is expected that the fabricated aptasensor will become an excellent diagnostic platform for IL-17RA-mediated diseases.
MeSH

DOI
10.1016/j.bios.2016.02.050
PMID
26921556
Appears in Collections:
Journal Papers > Hospital > Clinical Trial Center
Journal Papers > School of Medicine / Graduate School of Medicine > Allergy
Ajou Authors
김, 승현  |  박, 해심
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