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Preconditioning of mesenchymal stem cells with low-intensity ultrasound for cartilage formation in vivo.

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dc.contributor.authorCui, JH-
dc.contributor.authorPark, SR-
dc.contributor.authorPark, K-
dc.contributor.authorChoi, BH-
dc.contributor.authorMin, BH-
dc.date.accessioned2011-03-04T01:58:23Z-
dc.date.available2011-03-04T01:58:23Z-
dc.date.issued2007-
dc.identifier.issn1076-3279-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/1535-
dc.description.abstractThe purpose of this study was to evaluate the benefits of in vitro preconditioning of mesenchymal stem cells (MSCs) using low-intensity ultrasound (US) in the induction of chondrogenic differentiation of MSCs in vivo. After rabbit bone marrow-derived MSCs were seeded onto a polyglycolic acid (PGA) scaffold, the PGA-MSCs constructs were divided into 4 subgroups: untreated control, low-intensity US group, transforming growth factor-beta [TGF]-treated group and low-intensity US/TGF group. The chondrocyte-seeded PGA construct served as a positive control. For 1 week before implantation, the low-intensity US groups were subjected to ultrasound treatment for 20 min daily at an intensity of 200 mW/cm(2). The TGF groups were treated with 10 ng/mL TGF-beta1. The cells were then implanted into the nude mouse subcutaneously. Retrieved 1, 2, 4, and 6 weeks after implantation, each construct underwent gross examination, histology, biochemical assays, mechanical testing, and reverse transcriptase polymerase chain reaction (RT-PCR). Substantial size reduction and blood invasion were found much earlier in the groups that did not undergo low-intensity US than in those that did. Safranin O/Fast green staining revealed that the chondrogenic differentiation of MSCs was more widespread throughout the constructs in the low-intensity US groups. In the biochemical and mechanical analyses, the low-intensity US and low-intensity US/TGF groups were significantly better in forming hyaline cartilage-like tissue by 4 weeks than the non-low-intensity US groups. Presented by von Kossa staining, the development of osteogenic phenotypes was highly suppressed until 4 weeks in the low-intensity US groups, along with compressive strength comparable to the positive control. In the RT-PCR analysis before implantation, the messenger RNA levels of Sox-9, aggrecan, and tissue inhibitors of metalloproteinase-2 were higher in the low-intensity US groups, while those of type I and type X collagens and matrix metalloproteinase-13 were higher in the non-low-intensity US groups. Blood invasion into the constructs was also considerably hindered in the low-intensity US groups. These results strongly indicate that low-intensity US preconditioning in vitro could be an effective cue to upregulate chondrogenic differentiation of MSCs in vivo.-
dc.language.isoen-
dc.subject.MESHAnimals-
dc.subject.MESHCartilage-
dc.subject.MESHCell Differentiation-
dc.subject.MESHCell Proliferation-
dc.subject.MESHCells, Cultured-
dc.subject.MESHChondrogenesis-
dc.subject.MESHFemale-
dc.subject.MESHMesenchymal Stem Cells-
dc.subject.MESHOrgan Culture Techniques-
dc.subject.MESHRabbits-
dc.subject.MESHRadiation Dosage-
dc.subject.MESHSonication-
dc.subject.MESHTissue Engineering-
dc.titlePreconditioning of mesenchymal stem cells with low-intensity ultrasound for cartilage formation in vivo.-
dc.typeArticle-
dc.identifier.pmid17518569-
dc.contributor.affiliatedAuthor최, 병현-
dc.contributor.affiliatedAuthor민, 병현-
dc.type.localJournal Papers-
dc.identifier.doi10.1089/ten.2006.0080-
dc.citation.titleTissue engineering-
dc.citation.volume13-
dc.citation.number2-
dc.citation.date2007-
dc.citation.startPage351-
dc.citation.endPage360-
dc.identifier.bibliographicCitationTissue engineering, 13(2). : 351-360, 2007-
dc.identifier.eissn1557-8690-
dc.relation.journalidJ010763279-
Appears in Collections:
Journal Papers > Research Organization > Cell Therapy Center
Journal Papers > School of Medicine / Graduate School of Medicine > Orthopedic Surgery
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