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A case of the false-negative D phenotype in a neonate with a strongly positive direct antiglobin test rest

Other Title
신생아에서 직접항글로불린 강양성을 보인 D 혈액형 위음성 1예
Jung, YS | Lim, YA
Laboratory Medicine Online, 6(1). : 45-49, 2016
Journal Title
Laboratory Medicine Online
Accurate D antigen blood typing is needed owing to the clinical importance of the Rh blood group. We describe a female infant who was suspected to suffer from Rh incompatible hemolytic disease of the newborn, and who showed a strong positive direct antiglobin test (DAT) result and false red blood cell (RBC) agglutination in D typing. Using chloroquine dissociation of IgG, we confirmed that the antibodies coating her RBCs were of anti-D type. D typing with 0.8% RBC suspensions in saline using saline gel cards showed 2+ RBC agglutinations. After increasing the incubation time of dissociation by chloroquine for up to 4 hr, the dissociated RBCs began to show agglutination in both the tube technique (2+) and the gel card technique (4+) for D typing, although the DAT rest was still positive. Therefore, in order to prevent mistyping as a false-negative D blood group, whenever the D blood typing of a patient with a strong positive DAT rest does not show RBC agglutination, retesting of the D blood typing is recommended by using saline-suspended RBCs or dissociated RBCs.

Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Laboratory Medicine
Ajou Authors
임, 영애
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