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An in vitro Model of Neuron-macrophage Interaction to Generate Macrophages with Neurite Outgrowth Properties

Authors
Yun, HJ | Kim, BS
Citation
Bio-protocol, 6(22). : e2012-e2012, 2016
Journal Title
Bio-protocol
ISSN
2331-8325
Abstract
Macrophages are known to play beneficial roles in axon regeneration after nerve injury. To develop an in vitro model in which injury signals can elicit pro-regenerative macrophage activation, we established co-cultures consisting of adult dorsal root ganglia sensory neurons and peritoneal macrophages and added cAMP analogue dibutyryl cAMP. The conditioned medium collected from the co-cultures exhibited robust neurite outgrowth activities. The neurite outgrowth activities were almost completely abrogated by addition of minocycline, a macrophage deactivator, indicating that factors responsible for neurite outgrowth are produced by activated macrophages.
DOI
10.21769/BioProtoc.2012
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Brain Science
Ajou Authors
김, 병곤
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