Cited 0 times in Scipus Cited Count

Excretory and Secretory Proteins of Naegleria fowleri Induce Inflammatory Responses in BV-2 Microglial Cells

DC Field Value Language
dc.contributor.authorLee, J-
dc.contributor.authorKang, JM-
dc.contributor.authorKim, TI-
dc.contributor.authorKim, JH-
dc.contributor.authorSohn, HJ-
dc.contributor.authorNa, BK-
dc.contributor.authorShin, HJ-
dc.date.accessioned2018-07-27T00:52:17Z-
dc.date.available2018-07-27T00:52:17Z-
dc.date.issued2017-
dc.identifier.issn1066-5234-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/15595-
dc.description.abstractNaegleria fowleri, a free-living amoeba that is found in diverse environmental habitats, can cause a type of fulminating hemorrhagic meningoencephalitis, primary amoebic meningoencephalitis (PAM), in humans. The pathogenesis of PAM is not fully understood, but it is likely to be primarily caused by disruption of the host's nervous system via a direct phagocytic mechanism by the amoeba. Naegleria fowleri trophozoites are known to secrete diverse proteins that may indirectly contribute to the pathogenic function of the amoeba, but this factor is not clearly understood. In this study, we analyzed the inflammatory responses in BV-2 microglial cells induced by excretory and secretory proteins of N. fowleri (NfESP). Treatment of BV-2 cells with NfESP induced the expression of various cytokines and chemokines, including the proinflammatory cytokines IL-1alpha and TNF-alpha. NfESP-induced IL-1alpha and TNF-alpha expression in BV-2 cells were regulated by p38, JNK, and ERK MAPKs. NfESP-induced IL-1alpha and TNF-alpha production in BV-2 cells were effectively downregulated by inhibition of NF-kB and AP-1. These results collectively suggest that NfESP stimulates BV-2 cells to release IL-1alpha and TNF-alpha via NF-kB- and AP-1-dependent MAPK signaling pathways. The released cytokines may contribute to inflammatory responses in microglia and other cell types in the brain during N. fowleri infection.-
dc.language.isoen-
dc.subject.MESHAmoeba-
dc.subject.MESHAnimals-
dc.subject.MESHAntigens, Protozoan-
dc.subject.MESHCell Death-
dc.subject.MESHCell Line-
dc.subject.MESHCell Survival-
dc.subject.MESHCells, Cultured-
dc.subject.MESHCentral Nervous System Protozoal Infections-
dc.subject.MESHChemokines-
dc.subject.MESHCytokines-
dc.subject.MESHHost-Pathogen Interactions-
dc.subject.MESHInflammation-
dc.subject.MESHInterleukin-1beta-
dc.subject.MESHMice-
dc.subject.MESHMicroglia-
dc.subject.MESHMitogen-Activated Protein Kinase Kinases-
dc.subject.MESHNF-kappa B-
dc.subject.MESHNaegleria fowleri-
dc.subject.MESHProtozoan Proteins-
dc.subject.MESHTrophozoites-
dc.subject.MESHTumor Necrosis Factor-alpha-
dc.titleExcretory and Secretory Proteins of Naegleria fowleri Induce Inflammatory Responses in BV-2 Microglial Cells-
dc.typeArticle-
dc.identifier.pmid27480446-
dc.contributor.affiliatedAuthor손, 혜진-
dc.contributor.affiliatedAuthor신, 호준-
dc.type.localJournal Papers-
dc.identifier.doi10.1111/jeu.12350-
dc.citation.titleThe Journal of eukaryotic microbiology-
dc.citation.volume64-
dc.citation.number2-
dc.citation.date2017-
dc.citation.startPage183-
dc.citation.endPage192-
dc.identifier.bibliographicCitationThe Journal of eukaryotic microbiology, 64(2). : 183-192, 2017-
dc.embargo.liftdate9999-12-31-
dc.embargo.terms9999-12-31-
dc.identifier.eissn1550-7408-
dc.relation.journalidJ010665234-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Microbiology
Files in This Item:
There are no files associated with this item.

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse