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Effects of Adenoviral Gene Transduction on the Stemness of Human Bone Marrow Mesenchymal Stem Cells

Authors
Marasini, S | Chang, DY  | Jung, JH | Lee, SJ | Cha, HL | Suh-Kim, H  | Kim, SS
Citation
Molecules and cells, 40(8). : 598-605, 2017
Journal Title
Molecules and cells
ISSN
1016-84780219-1032
Abstract
Human mesenchymal stem cells (MSCs) are currently being evaluated as a cell-based therapy for tissue injury and degenerative diseases. Recently, several methods have been suggested to further enhance the therapeutic functions of MSCs, including genetic modifications with tissue- and/or disease-specific genes. The objective of this study was to examine the efficiency and stability of transduction using an adenoviral vector in human MSCs. Additionally, we aimed to assess the effects of transduction on the proliferation and multipotency of MSCs. The results indicate that MSCs can be transduced by adenoviruses in vitro, but high viral titers are necessary to achieve high efficiency. In addition, transduction at a higher multiplicity of infection (MOI) was associated with attenuated proliferation and senescence-like morphology. Furthermore, transduced MSCs showed a diminished capacity for adipogenic differentiation while retaining their potential to differentiate into osteocytes and chondrocytes. This work could contribute significantly to clinical trials of MSCs modified with therapeutic genes.
MeSH

DOI
10.14348/molcells.2017.0095
PMID
28835020
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Anatomy
Ajou Authors
김, 성수  |  서, 해영  |  장, 다영
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